骨性关节炎中lncRNA MEG3的表达及其与VEGF的相关性分析

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Abstract Objective To determine the expressions of long non-coding RNA MEG3 (lncRNA MEG3) and vascular endothelial growth factor (VEGF) in the articular cartilage tissues of osteoarthritis (OA), and to analyze the correlation between the lncRNA MEG3 and VEGF. Methods The knee articular cartilage tissues from 40 cases of primary OA and 20 healthy volunteers were collected from January 2014 to December 2015 in Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region. After the safranin O staining, the OA patients were grouped according to the improved score criterion of Mankin. Real-time PCR assay was applied to detect the expressions of lncRNA MEG3 and VEGF mRNA in the knee articular cartilage tissues, and the ELISA assay was utilized to determine the protein level of VEGF. The correlation between the lncRNA MEG3 and VEGF was analyzed by the Pearson. Results The real-time PCR results showed that compared to the normal articular cartilage tissues, the lncRNA MEG3 was significantly downregulated in the OA articular cartilage tissues (P < 0.05), and the higher degree of degradation, the lower of lncRNA MEG3 (P < 0.05). Moreover, the mRNA and protein levels of VEGF were significantly upregulated in the OA articular cartilage tissues (P < 0.05), and the higher degree of degradation, the higher of VEGF (P < 0.05). The Pearson results showed that there was a negative relationship between the lncRNA MEG3 and VEGF (r = -0.466, P < 0.05). Conclusion lncRNA MEG3 was decreased in the articular cartilage tissues of OA, and lncRNA MEG3 was negatively correlated with the VEGF. lncRNA MEG3 may be involved in primary OA development through the regulation of angiogenesis.

Key words： Knee osteoarthritis Long non-coding RNA MEG3 Vascular endothelial grouth factor Correlation

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	LIU Zhenfeng LIANG Zhiquan DENG Yingjie FANG Rui MENG Qingcai

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LIU Zhenfeng LIANG Zhiquan DENG Yingjie FANG Rui MENG Qingcai. Expression of lncRNA MEG3 in the osteoarthritis and its correlation with VEGF[J]. 中国医药导报, 2018, 15(16): 8-12.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I16/8

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