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| Study on the differences of the expression of vascular endothelial growth factor and the activity of NK cells in lung cancer mice with different behavior characteristics |
| ZANG Fucai1 LIU Mingyue2 LIU Min1 TANG Wei1 |
1.Neurology Department, Xinhua Hospital Affiliated to Dalian University, Liaoning Province, Dalian 116021, China;
2.the Second Radiotherapy Department, Handan Central Hospital, Hebei Province, Handan 056000, China |
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Abstract Objective To investigate the differences of the expression of vascular endothelial growth factor (VEGF) and the activity of NK cells in lung cancer mice with different behavior characteristics. Methods Eighty male Balb/c mice were taken open field test to screen high active group and low active group, with 20 mice in each group. The logarithmic phase LLC cells were inoculated in the right axillary subcutaneous of high active group and low active group to establish lung cancer model. After 14 days under the same feeding condition, the killing activity of spleen NK cells in the two groups was detected by MMT method. The long and short diameters of tumor were detected by vernier caliper, the tumor volume was calculated, and the tumor weight was measured by electronic scales. Immunohistochemistry method was used to detect the expression of VEGF in the two groups. Results The killing activity of NK cells in the high active group was (19.73±3.55)%, which was significantly higher than that of low active group [(16.47±3.29)%], the difference was statistically significant (P ＜ 0.05). The tumor volume of high active group was (0.78±0.14) cm3, which was smaller than that of low active group [(0.94±0.24) cm3], the difference was statistically significant (P ＜ 0.05). The tumor weight of high active group was (0.84±0.19) g, which was lower than that of low active group [(1.00±0.22) g], the difference was statistically significant (P ＜ 0.05). The positive expression rate of VEGF in the high active group was 85.0%, which was lower than that of low active group (95.0%), there was statistically significant difference (P ＜ 0.05). Conclusion Different activity degrees of tumor-bearing mice have different growth rates of lung cancer tissue, which may be connected with the activity of NK cells and the expression of VEGF protein in tumor tissue.
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