Apelin-13（F13A）对M2型肿瘤相关巨噬细胞诱导的乳腺癌细胞侵袭的影响及机制

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Abstract Objective To investigate effect of putative receptor protein related to the angiotensin receptor AT1 (APJ) antagonist Apelin13 (F13A) on the polarization of tumor-associated macrophages (TAM) and the invasion induced by M2 type macrophages in breast cancer cells line MCF-7 cells. Methods Macrophage RAW264.7 cells were treated with IL-13 (10 ng/mL) for 24 h to induce M2 type polarization (M2RAW) and the cells were treated with Apelin-13(F13A) (10.00 pm/mL) for 24 h. RAW and MCF-7 cells were co-cultured for 48 h in non-contact manner. The proliferation of RAW and MCF-7 was detected by MTT. Levels of transforming growth factor-β (TGF-β) and interleukin-10 (IL-10) in culture medium were tested by ELISA. Expression of marker protein of M2 type polarization arginase 1 (Arg-1) were measured by Western blot. Migration and invasiveness of MCF-7 cells were tested by scratch test and Transwell method. Results Compared with RAW group, levels of TGF-β and IL-10 and expression of Arg-1 in M2RAW group were significantly increased, the differences were statistically significant (P < 0.05). Compared with M2RAW group, levels of TGF-β and IL-10 and expression of Arg-1 in M2RAW+Apelin-13 (F13A) group were significantly decreased, the differences were statistically significant (P < 0.05). Compared with RAW+MCF-7 group, scratch area was significantly decreased, the number of cell through the membrane was significantly increased in M2RAW+MCF-7 group, the differences were statistically significant (P < 0.05). Compared with M2RAW+MCF-7 group, scratch area was significantly increased, the number of cell through the membrane was significantly decreased in Apelin-13(F13A)+M2RAW+MCF-7 group, the differences were statistically significant (P < 0.05). Conclusion Apelin13 (F13A) inhibits the polarization of tumor related macrophages and the invasion induced by M2 type macrophages in breast cancer cells line MCF-7 cells.

Key words： Putative receptor protein related to the angiotensin receptor AT1 Antagonists Mammary cancer Tumor invasion Tumor-associated macrophages M2 type polarization

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	PENG Chuanzhen SONG Zhaolu LI Kaiyan WU Xiao LENG Ning

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PENG Chuanzhen SONG Zhaolu LI Kaiyan WU Xiao LENG Ning. Effect of putative receptor protein related to the angiotensin receptor AT1 antagonist Apelin13 (F13A) on the invasion induced by M2 type tumor related macrophages in breast cancer cells line MCF-7 cells and probable mechanism[J]. 中国医药导报, 2018, 15(15): 13-17,42.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I15/13

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