|
|
|
| Effect of water extraction of green tea on survival and proliferation of PC12 cells |
| CAI Zhenlu1 FENG Yunran2 LI Xingxing1 LI Mu3▲ LV Haixia1 |
1.Institute of Neurobiology, Xi′an Jiaotong University Health Science Center, Shaanxi Province, Xi′an 710061, China;
2.the Affiliated Middle School of Shaanxi Normal University, Shaanxi Province, Xi′an 710061, China;
3.Department of Obstetrics and Gynecology, the Second Hospital of Xi′an Jiaotong University, Shaanxi Province, Xi′an 710004, China |
|
|
|
Abstract Objective To investigate the effect of green tea on the survival and proliferation of neural cells during development. Methods PC12 cells were selected as the cell model for toxicological analysis, green tea leaves were boiled in distill water of 100℃ for three times, tea solution was then filtered and freeze dried. The powder was dissolved with sterilized pure water. DMEM medium was applied to make different concentrations of tea extraction (diluer for 100, 200 400, 800, and 1600 times) before use. The morphology of PC12 cells was observed, cell viability and apoptosis were detected by CCK-8 assay and flow cytometry assay after treatment. The proliferation of PC12 was detected by Ki67 immunostaining. Results Compared with low concentration (diluer 800 and 1600 times), after treated with green tea extraction in high concentration, PC12 cell morphology changed dramatically and cell viability was decreased significantly (P < 0.05). Proliferation of PC12 also declined dramatically, as showed by the reduced number of Ki67+ cells (P < 0.05). While the apoptosis of PC12 had no statistically significant difference compared with control group (P > 0.05). Conclusion Green tea in high concentration, so it is recommended to reduce the drinking of strong green teadurn pregancy could reduce the survival and proliferation of PC12 cells
|
|
|
|
|
|
[1] Zhang J，Cai S，Li J，et al. Neuroprotective Effects of Theaflavins Against Oxidative Stress-Induced Apoptosis in PC12 Cells [J]. Neurochem Res，2016，41（12）：3364-3372.
[2] 李慎新，曹新志.几种茶叶中抗氧化性物质的比较研究[J].四川理工学院学报：自科版，2009，22（4）：82-84.
[3] Butt MS，Ahmad RS，Sultan MT，et al. Green tea and anticancer perspectives：updates from last decade [J]. Crit Rev Food Sci Nutr，2015，55（6）：792-805.
[4] Hayakawa S，Saito K，Miyoshi N，et al. Anti-Cancer Effects of Green Tea by Either Anti- or Pro- Oxidative Mechanisms [J]. Asian Pac J Cancer Prev，2016，17（4）：1649-1654.
[5] Zink A，Traidl-Hoffmann C. Green tea in dermatology—myths and facts [J]. J Dtsch Dermatol Ges，2015，13（8）：768-775.
[6] Kataoka S，Norikura T，Sato S. Maternal green tea polyphenol intake during lactation attenuates kidney injury in high-fat-diet-fed male offspring programmed by maternal protein restriction in rats [J]. J Nutr Biochem，2018，56：99-108.
[7] Menezes J，Neves BH，Souza M，et al. Green tea protects against memory deficits related to maternal deprivation [J]. Physiol Behav，2017，182：121-127.
[8] Zhong J，Xu C，Reece EA，et al. The Green Tea Polyphenol EGCG Alleviates Maternal Diabetes-Induced Neural Tube Defects by Inhibiting DNA Hypermethylation [J]. Am J Obstet Gynecol，2016，215（3）：368.e1-368.
[9] Ye R，Ren A，Zhang L，et al. Tea Drinking as A Risk Factor for Neural Tube Defects in Northern China [J]. Epidemiology，2011，22（4）：491-496.
[10] Ajarem J，Rashedi GA，Mohany M，et al. Neurobehavioral changes in mice offspring exposed to green tea during fetal and early postnatal development [J]. Behav Brain Funct，2017，13（1）：10.
[11] Zhang JC，Xu H，Yuan Y，et al. Delayed Treatment with Green Tea Polyphenol EGCG Promotes Neurogenesis After Ischemic Stroke in Adult Mice [J]. Mol Neurobiol，2017， 54（5）：3652-3664.
[12] 邓凤君，肖凌，杨吟宇，等.茶多酚对过氧化氢损伤PC12细胞抗氧化酶系统的影响[J].中国医药导报，2017， 14（7）：20-23.
[13] 张梁，韩煜晖，秦金花.发酵茶叶水浸出物对PC12神经细胞保护作用[J].食品研究与开发，2014（4）：1-4.
[14] Greene LA，Tischler AS. Establishment of a Noradrenergic Conal Line of Rat Adrenal Pheochromocytoma Cells Which Respond to Nerve Growth Factor [J]. PNAS USA，1976，73（7）：2424-2428.
[15] 宋明旭，杨吉成，王蔚平.谷氨酸诱导的PC12细胞损伤的研究[J].苏州科技学院学报：自然科学版，2006，23（1）：62-65.
[16] 陈娜娜，雷鸣，周清娣，等.基于AMPK/Sirt1 信号通路研究黔北绿茶水提物对过氧化氢诱导的PC12细胞氧化损伤的保护作用[J].中药药理与临床，2017，33（6）：80-84.
[17] 马开利，宋连昆，苑玉和，等.稳定表达α-Synuclein4种选择性剪接异构体的PC12细胞对神经毒性剂MPP+的敏感性研究[J].中国药理学通报，2013，29（6）：814-817.
[18] 钱晓东，袁玉梅，曹恒斌，等.依托考昔对低氧缺糖诱导PC12细胞损伤的保护作用[J].中国医药导报，2010，7（9），13-14.
[19] 姚娟，马慧萍，杨燕，等.缺氧环境对PC12细胞损伤及HIF-1 mRNA表达水平的影响[J].中国药理学通报，2011， 27（2）：162-166.
[20] 戚之琳，刘银华，齐世美，等.红景天苷通过抑制NOX2-ROS-MAPKs信号途径保护H2O2诱导的PC12细胞凋亡[J].南方医科大学学报，2017，37（2）：178-183. |
|
|
|
