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| A case of tri-allele in TPOX was detected using Goldeneye 20A Kit for the first time |
| ZHOU Danhua1,2* HU Xijie1,2* ZHANG Tao1,2 WAN Weihong1,2 YU Jian2 LIU Zulin1,2 YU Limei1,2 |
1.Key Laboratory of Cell Engineering in Guizhou Province, Guizhou Province, Zunyi 563003, China;
2.Centre of Forensic Medicine, the Affiliated Hospital of Zunyi Medical University, Guizhou Province, Zunyi 563003, China |
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Abstract This article reports one case of tri-allele detected in the TPOX locus of a child in duos. The Goldeneye 20A kit was used for detecting. The 3500DX genetic analyzer was used for electrophoresis and Genemapper genotyping software was used confirm its genotyping (8, 9, 11). When the tri-allelic gene is detected in the paternity test, under the premise of eliminating contamination, at least two kits are required to confirm the test to ensure the reliability of the identification and PI calculation. In order to improve the accuracy of the case report, the tri-allele test, the peak type analysis, the classification and judgment and the confirmation should be handled with care so that provides basic information for the analysis of the possible mechanisms of tri-allele.
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[1] Crouse CA，Rogers S，Amiott E，et al. Analysis and interpretation of short tandem repeat microvariants and three-banded allele patterns using multiple allele detection systems [J]. J Forensic Sci，1999，4（1）：87-94.
[2] Butler JM，Dennis J. Reeder （NIST），[217-03-03] Short Tandem repeat DNA Internet Database. http：//strbase.nist.gov.
[3] 贾雲舒，钱水，周凤蕾，等.D13S317基因座三等位基因遗传学分析[J].中国法医学杂志，2015，30（3）：312-313.
[4] 刘亚举，岳俊涛.D21S11和Penta D基因座STR分型异常2例[J].刑事技术，2014，39（5）：62-63.
[5] 周雪，王鑫，杨俊.DYF387S1基因座检出三等位基因1例[J].中国法医学杂志，2016，31（5）：525.
[6] Lane AB. The nature of tri-allelic TPOX genotypes in African populations [J]. Forensic Sci Int Genet，2008，2（2）：134-137.
[7] 刘秋玲，赖运科，吕德坚，等.TPOX基因座遗传多态性及三等位基因研究[J].证据科学，2010，18（6）：748-749.
[8] Pican?觭o JB，Raimann PE，Rodenbusch R，et al. Identification of the third/extra allele for forensic application in cases with TPOX tri-allelic pattern [J]. Forensic Sci Int Genet，2015，16：88-93.
[9] Pican?觭o JB，Raimann PE，Paskulin GA，et al. Tri-allelic pattern at the TPOX locus：A familial study [J]. Gene，2014， 535（2）：353-358.
[10] 蒋涵玮，李涛，樊岁兴，等.减数分裂染色体的行为及其分子基础[J].中国科学：生命科学，2017，47（1）：16-25.
[11] Chantot-Bastaraud S，Stratmann S，Brioude F，et al. Formation of upd（7） mat by trisomic rescue：SNP array typing provides new insights in chromosomal nondisjunction [J]. Mol Cytogenet，2017，10（1）：28-34.
[12] Chang YL，Yi WP，Chao AS，et al. Monozygotic twins discordant for trisomy 21：Discussion of etiological events involved [J]. Taiwan J Obstet Gynecol，2017，56（5）：681-685.
[13] Tao Y，Gross N，Fan X，et al. Identification of novel enriched recurrent chimeric COL7A1-UCN2 in human laryngeal cancer samples using deep sequencing [J]. BMC Cancer，2018，18（1）：248.
[14] Dziegiel MH，Hansen MH，Haedersdal S，et al. Blood Chimerism in Dizygotic Monochorionic Twins During 5 Years Observation [J]. Am J Transplant，2017，17（10）：2728-2732.
[15] Van BY，Wolffenbuttel KP，Brüggenwirth HT，et al. Multiparameter Investigation of a 46，XX/46，XY Tetragametic Chimeric Phenotypical Male Patient with Bilateral Scrotal Ovotestes and Ovulatory Activity [J]. Sex Dev，2018， 12（1-3）：145-154.
[16] Mascarell L，Auger R，Kanellopoulos JM，et al. Triallelic patterns in STR loci used for paternity analysis：evidence for a duplication in chromosome 2 containing the TPOX STR locus [J]. Forensic Sci Int，2006，164（1）：3-9.
[17] 刘芳，任贺，陈冲，等.三联体常染色体STR三带型基因座父权指数计算[J].刑事技术，2017，42（6）：471-475.
[18] Jiao H，Ren H，Yang Y，et al. Tri-allelic patterns of STRs and partially homologous non-sister chromatid crossover observed in a parentage test [J]. Leg Med （Tokyo），2017， 30：34-37.
[19] Jiang E，Pan J，Han M，et al. Tri-allelic patterns at the D7S820 locus detected in two generations of a Chinese family [J]. Int J Legal Med，2016，130（1）：23-26.
[20] 刘亚举，刘开会，高林波，等.STR基因座中检出三等位基因1例[J].法医学杂志，2008，24（5）：399-400.
[21] Wang LF，Yang Y，Zhang XN，et al. Tri-allelic pattern of short tandem repeats identifies the murderer among identical twins and suggests an embryonic mutational origin [J]. Forensic Sci Int Genet，2015，16（2）：239-245. |
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