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| Detection of antimutagenic effect of Genistein |
| ZOU Siying1 ZHANG Lishi2 |
| 1.Sichuan Center for Disease Control and Prevention, Sichuan Province, Chengdu 610041, China; 2.West China School of Public Health, Sichuan University, Sichuan Province, Chengdu 610041, China |
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Abstract [Abstract] Objective To evaluate the antimutagenic effect of Genistein. Methods L5178Y cells were cultivated, and grouped according to the different processing methods, the L5178Y cells were respectively exposed to the Genistein concentrations of 0.078, 0.156, 0.312, 0.625 μg/mL with 5 μg/mL methyl methanesulfonate （MMS）or 0.5 μg/mL mitomycin C (MMC) (positive mutation group), 5 μg/mL MMS or 0.5 μg/mL MMC (positive mutation control group), 0.5% DMSO (solvent control group), water (negative control group), 5 μg/mL MMS or 0.5 μg/mL MMC and 100 μg/mL VitC + 5 μg/mL MMS or 0.5 μg/mL MMC (anti mutagenesis positive control group). The antimutagenic effect of genistein were determined by the mouse lymphoma Assay in L5178Y cells, in the evaluation of antimutagenic effect three kinds of treatment (pre-treatment, silmutaneous-treatment and post-treatment) were used to detect the antimutagenic effects of Genistein and puerarin against gene mutations induced by MMS and MMC. Determination of mutant frequency of tk locus (TFT) was performed according to the microcell method. Results In pre-treatment and post-treatment, at all the tested concentrations the total mutation frequencies were lower than the MMS or MMC positive mutation control group the differences were statistically significant (P < 0.01). In simultaneous treatment, the total mutation frequencies at concentrations of 0.312, 0.625 μg/mL were lower than MMS and MMC positive mutation control group, the differences were statistically significant (P ＜ 0.01). Conclusion Under the three kinds of treatment, Genistein shows a good role for preventing TK gene mutation induced by MMS or MMC, pre-treatment has the strongest inhibitory effect, had a good application and development prospect.
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