壳聚糖薄膜诱导间充质干细胞形成球体的研究进展及临床应用前景

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Abstract Mesenchymal stem cells (MSCs) with a wide range of sources and multipotential differentiation are thought as a new hope in the field of tissue repair and reconstruction. However, recent research shows that traditional adherent culture changes the initial growing environment of MSCs, leading to self-differentiation, stemness recession and other problems, which brings a negative effect to the treatment after in vivo transplantation. And the evidence suggests that, compared with the traditional adherent culture, the 3D-sphere culture plays a significant role in promoting the biological activity of MSCs. Chitosan membrane (CM) culture is a new type of biomaterial substrate-mediated multicellular spheroid cultivation for MSCs. This review discusses the CM culture of MSCs mainly from the change of morphology, biological functions and potential mechanisms, as well as its potential in clinical application.

Key words： Mesenchymal stem cells 3D-sphere Chitosan membrane Stemness

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	LI Huajie XU Xiongfeng QIU Bo

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LI Huajie XU Xiongfeng QIU Bo. Research progress and clinical application prospect in 3D-sphere culture of mesenchymal stem cell with chitosan membrane[J]. 中国医药导报, 2018, 15(14): 21-25.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I14/21

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