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| Laboratory animal research on the regulatory effects of recombinant growth hormone on lipid metabolism |
| LUO Yanfei Bulibur·Bahr Mireguli·Mimiti CAO Chen LIANG Ling |
| Department of Pediatrics, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China |
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Abstract Objective To study the effect of growth hormone on lipid metabolism in animal experiments. Methods Be used 7-day-old mice to fed with a fat-increasing diet and a free diet respectively for 7 day, then super-high fat diet for 4 weeks to build obesity model, and were randomly divided into four groups. Each group was given rhGH 0.0, 0.1, 0.2, 0.3 U /(kg·d) intramuscularly and fed under the same conditions for two weeks. The changes of body weight, serum triglyceride, cholesterol, high and low density lipoprotein, leptin and insulin growth factor-1 were observed after one week and two weeks. Results Before and after the experiment, the indexes of mice were not significantly different (P > 0.05). The body weight, TG, TC, Leptin and LDL-C decreased significantly at 1 week and 2 weeks after injection, and decreased with the increase of time (P < 0.05). IGF-1 increased at 1 week and 2 weeks after injection, and increased with the increase of injection time (P < 0.05). There was no significant change in HDL-C before and after injection (P > 0.05). Body weight, TG, TC, Leptin and LDL-C increased with the increase of growth hormone concentration (P < 0.05). IGF-1 increased with the increase of growth hormone concentration (P < 0.05). Conclusion A certain dose of growth hormone has a certain regulation on lipid hormones, can improve the level of lipid metabolism, and with the dose increase, the more obvious effect.
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[1] Roemmler J，Kuenkler M，Schneider HJ，et al. Comparison of glucose and lipid metabolism and bone mineralization in patients with growth hormone deficiency with and without long-term growth hormone replacement [J]. Metabolism，2010，59（3）：350-358.
[2] Vijayakumar A，Novosyadlyy R，Wu Y，et al. Biological effects of growth hormone on carbohydrate and lipid metabolism [J]. Growth Horm IGF Res，2010，20（1）：1-7.
[3] 李彩霞，刘丽蓉，邵珊，等.重组生长激素对肾病综合征患者血脂代谢的影响[J].山西医科大学学报，2009，40（9）：828-831.
[4] Jorgensen AP，Fougner KJ，Ueland T，et al. Favorable long-term effects of growth hormone replacement therapy on quality of life，bone metabolism，body composition and lipid levels in patients with adult-onset growth hormone deficiency [J]. Growth Horm IGF Res，2011，21（2）：69-75.
[5] 李枋沄，薛松梅，单胜华，等.单纯性肥胖儿童生存质量的研究进展[J].中国妇幼保健，2017，32（9）：2047-2049.
[6] Liu Z，Cordoba-Chacon J，Kineman RD，et al. Growth Hormone Control of Hepatic Lipid Metabolism [J]. Diabetes，2016，65（12）：3598-3609.
[7] Cenci MC，Soares DV，Spina LD，et al. Comparison of two dose regimens of growth hormone （GH） with different target IGF-1 levels on glucose metabolism，lipid profile，cardiovascular function and anthropometric parameters in gh-deficient adults[J]. Growth Horm IGF Res，2012，22（3-4）：116-121.
[8] Ren S，Nie Y，Wang A. Effects of recombinant human growth hormone in the treatment of dwarfism and relationship between IGF-1，IGFBP-3 and thyroid hormone[J]. Exp Ther Med，2016，12（6）：3579-3582.
[9] Keane J，Tajouri L，Gray B. Recombinant human growth hormone and insulin-like growth factor-1 do not affect mitochondrial derived highly reactive oxygen species production in peripheral blood mononuclear cells under conditions of substrate saturation in-vitro [J]. Nutr Metab （Lond），2016，13（1）：45.
[10] 胡孝跃，杨硕，孙泽，等.浅述高脂饲料构建大鼠肥胖模型过程中需注意问题[J].临床医药文献电子杂志，2017， 37（1）：7151.
[11] 朱磊，路瑛丽，冯连世，等.不同配方高脂饲料构建SD大鼠肥胖模型的实验研究[J].中国运动医学杂志，2016， 7（1）：642-647.
[12] 史博，李洪俊.生长激素治疗Turner综合征患儿糖脂代谢临床疗效分析[J].中国保健营养，2017，27（4）：65.
[13] 迟雅琳.单纯性肥胖儿童血清瘦素水平及其与糖脂代谢异常的研究[J].世界最新医学信息文摘：电子版，2014， 15（4）：25-26.
[14] 郑方圆，王雪梅，王新利.重组人生长激素治疗对特发性矮小儿童的糖脂代谢及甲状腺功能的影响[J].中国当代儿科杂志，2014，12（1）：1236-1240.
[15] Sangiao-Alvarellos S，Vazquez MJ，Varela L，et al. Central ghrelin regulates peripheral lipid metabolism in a growth hormone-independent fashion[J]. Endocrinology，2009，150（10）：4562-4574.
[16] Yang HL，Feng M，Tan X，et al. The role of SOCS2 in recombinant human growth hormone （rhGH） regulating lipid metabolism in high-fat-diet-induced obesity mice[J]. Mol Biol Rep，2013，40（3）：2319-2326.
[17] 程向登，梁爽，胡艳艳，等.Prader-Willi综合征患儿下丘脑-垂体-靶腺轴功能及代谢的改变[J].山东大学学报：医学版，2017，55（3）：107-111.
[18] Baranowska B，Wolinska-Witort E，Martynska L，et al. The role of leptin in the regulation of pituitary hormones release [J]. Neuro Endocrinol Lett，2005，26（5）：459-462.
[19] 武苏，刘倩琦，顾威，等.儿童身材矮小与青春期激素及BMI的相关性[J].基因组学与应用生物学，2017，8（1）：3322-3327.
[20] 葛青玮，朱志颖.IGF-1、IGFBP3及其比值与生长激素缺乏型矮小症的相关性研究[J].临床和实验医学杂志，2017 （3）：255-257. |
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