|
|
|
| Effects of Shenyu Buxue Decotion on the Th17 cells and expression of its related IL-6, IL-17 and IL-23 expression in mice with aplastic anemia |
| ZENG Qing1 LU Haisong1 CHENG Weimin1 HE Ai1 DENG Dejun2 LAI Chunfeng3 |
1.Department of Hematology, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530023, China; 2.Graduate School, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 500001, China;
3.Department of Oncology, Yullin Traditional Chinese Medicine Hospital, Guangxi Zhuang Autonomous Region, Yulin 537000, China |
|
|
|
Abstract Objective To observe the effect of Shenyu Buxue Decotion on Th17 cells and the expression of IL-6, IL-17 and IL-23 in aplastic anemia (AA) model mice, and to explore its curative effect for AA. Methods Eighty clean-class BALB/c mice were divided into control group, model group, Shenyu Buxue Decotion group and Prednisone (positive control) group by random number table. The AA models were established by irradiated with 60CO-γ ray together with intraperitoneal injection of cyclophosphamide (CY) and chloramphenicol (CH). After AA models were successfully established, mice were given to medicines for 4 weeks. The changes of mice peripheral hemogram, Th17 cells and the expression levels of IL-6, IL-17 and IL-23, and the degree of bone marrow hyperplasia under light microscope were observed. Results Compared with the control group, the peripheral hemogram and bone marrow hyperplasia were decreased, while Th17 cells and expression of IL-6, IL-23 and IL-17 were increased in the model group, with statistically significant difference (P < 0.05). Compared with the model group, in the Shenyu Buxue Decotion group, the peripheral hemogram were increased, Th17 cells and the expression of IL-6, IL-23 and IL-17 were decreased, and bone marrow hyperplasia was improved, with statistically significant difference (P < 0.05). Conclusion Shenyu Buxue Decotion can improve the bone marrow hematopoietic function of AA mice, which may due to the inhibition of the T cell immune function related IL-6, IL-17 and IL-23.
|
|
|
|
|
|
[1] 孙纪元，王四旺，谢艳华，等.再生障碍性贫血的动物模型实验研究[J].中国实验动物学杂志，2000，10（4）：210-212.
[2] 訾芙玮.中药X方剂治疗小鼠再生障碍性贫血的实验研究[D].哈尔滨：哈尔滨师范大学，2012.
[3] 严苏纯，祝彼得，陈志伟.几种骨髓抑制性贫血小鼠模型的比较研究[J].成都中医药大学学报，2007，30（1）：31-34.
[4] 董玢，刘涓，娄利霞，等.补肾生血解毒方对再生障碍性贫血小鼠红细胞生成相关细胞因子表达的影响[J].北京中医药大学学报，2014，37（6）：397-409.
[5] 马昆.中医治疗再生障碍性贫血验案[N].中国中医药报，2017-12-25（005）.
[6] 胡晓梅，胡乃平，刘锋.周霭祥诊治再生障碍性贫血的学术思想[J].中医杂志，2011，52（9）：731-734.
[7] 张华平.中西医结合治疗慢性再生障碍性贫血临床研究[J].亚太传统医药，2017，13（4）：123-124.
[8] 刘春燕，邵宗鸿.再生障碍性贫血发病机制研究进展[J].中国实用内科杂志，2016，36（5）：345-349.
[9] 石岭，王运律.再生障碍性贫血中西医发病机制及其治疗进展[J].辽宁中医药大学学报，2014，16（5）：74-76.
[10] 任美华，翟声平.参附注射液治疗再生障碍性贫血模型小鼠的实验研究[J].中国现代药物应用，2013，7（14）：35-36.
[11] 中华医学会血液学分会红细胞疾病（贫血）学组.再生障碍性贫血诊断与治疗中国专家共识（2017年版）[J].中华血液学杂志，2017，38（1）：1-5.
[12] Shevach EM. Biological functions of regulatory T cells [J]. AdvImmunol，2011，112：137-176.
[13] El Mahgoub IR，Afify RA，Botros SK，et al. Immunoregulatory cytokines gene polymorphisms in Egyptian patients affected with acquired aplastic anemia [J]. Ann Hematol，2014，93（6）：923-929.
[14] 李军，黄可欣，陆培信，等.再生障碍性贫血患者外周血和骨髓中IL-17、IL-23、及TGF-β表达水平及其临床意义[J].吉林大学学报：医学版，2014，40（1）：146-149.
[15] 胡令彦，周永明，陈英坤，等.健脾补肾方对再生障碍性贫血患者外周血IL-6、IL-17和TGF-β1的影响[J].中医药信息，2013，30（5）：31-34.
[16] 张强，李庆，徐静玮，等.再生障碍性贫血患者T细胞亚群检测的临床意义[J].中国实验血液学杂志，2007，15（5）：1046-1049.
[17] 孙凤，李聪，初静文，等.补肾生血法治疗慢性再生障碍性贫血的临床观察及其对IL-23的影响[J].黑龙江中医药，2016，45（4）：15-16.
[18] 邓德君，曾清，赖春凤，等.再生障碍性贫血细胞免疫机制的研究进展[J].广西医学，2017，39（8）：1215-1218， 1224.
[19] 雷载权，张迁模.中华临床中药学（下卷）[M].北京：人民卫生出版社，1998.
[20] 曾清，程纬民，韦常丽，等.参萸补血方对皮肾阳虚型慢性再生障碍性贫血T细胞免疫机制的影响[J].四川中医，2007，25（10）：64-65.
[21] 曾清，程纬民.参萸补血方为主治疗慢性再生障碍性贫血30例[J].广西中医学院学报，2005，8（1）：18-19. |
|
|
|
