间充质干细胞协同BMP-2蛋白对造血干细胞增殖的影响

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Abstract Objective To explore the proliferation effect of bone morphogenetic protein (BMP)-2 on hematopoietic stem cell (HSC) cocultured with mesenchymal stem cell (MSC)． Methods MSCs were expanded to the third passage (P3) in the plastic dish; CD34+ cells were sorted by MACS Miltenyi Biotec. MSCs(P3) and HSCs were identified by FCM separately, and then MSC (P3) and HSC were co-cultured in the Transwell interfered with BMP-2 protein, four groups in the study: HSC group, HSC+BMP2 group, HSC+MSC group, HSC+MSC+BMP2 group. Cells were collected after 3 d to test HSC proliferation using the following methods：counting of the number, detecting of the total RNA, the mRNA and protein expression of Ki67 were detected by fluorescence qRT-PCR and immunofluorescence methods. Results The number of HSC, totel RNA, Ki67 expression of the HSC+BMP2 group was higher than HSC group, HSC+MSC group was higher than HSC group, HSC+MSC+BMP2 group was higher than HSC+MSC group (P < 0.05). Conclusion MSCs synergistic with BMP-2 can enhance MSC proliferation effect on HSC.

Key words： Hematopoietic stem cell Mesenchymal stem cell BMP-2 Proliferation

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	LI Shutan HUANG Chunlan

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LI Shutan HUANG Chunlan. The effect of bone marrow mesenchymal stem cell synergistic BMP-2 in the proliferation of hematopoietic stem cell[J]. 中国医药导报, 2018, 15(12): 4-7,24.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I12/4

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