僵蚕四草汤对咪喹莫特诱导银屑病样小鼠模型的治疗效果及机制研究

doi:10.20047/j.issn1673-7210.2023.06.06

Abstract
Figure/Table
References (26)
Related Citation (15)

Download: PDF (1540 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the therapeutic effect of Jiangcan Sicao Decoction on imiquimod-induced psoriasis mice model, and to discuss its mechanism. Methods Sixty SPF male BALB/c mice of six to eight weeks were divided into blank group, model group, positive drug control group, Jiangcan Sicao Decoction high-concentration, medium-concentration, and low- concentration groups, with ten mice in each group. Except blank group, the other five groups were treated with 5% Imiquimod Cream 62.5 mg on the back to prepare psorias-like mouse model. Meanwhile, Jiangcan Sicao Decoction high-concentration, medium-concentration, and low-concentration groups were given 20, 10, and 5 mg/kg suspension of Jiangcan Sicao Decoction by intragastric administration, respectively; positive drug control group was given 3.75 mg/kg of distilled water suspension of acitretin by gavage; blank group and model group were given normal saline intragastric administration, once per day for seven consecutive days. The skin area, histopathology, psoriasis area and severity index (PASI) score and serum levels of interleukin (IL)-17A, IL-23, tumor necrosis factor α (TNF-α), growth transforming factor β (TGF-β), and tumor necrosis factor-like weak apoptosis inducer (TWEAK) were compared in each group. Results PASI score in model group was higher than that in blank group (P＜0.05); PASI scores in Jiangcan Sicao Decoction high-concentration, medium-concentration, and low-concentration groups were lower than those in model group (P＜0.05); PASI score of Jiangcan Sicao Decoction medium-concentration group was lower than that of Jiangcan Sicao Decoction low-concentration group, and PASI score of Jiangcan Sicao Decoction high-concentration was higher than that of Jiangcan Sicao Decoction medium-concentration group (P＜0.05). On the 7th day of modeling administration, blank group showed no abnormal symptoms, and the other groups showed different degrees of erythema, scale, and infiltration; compared with model group, the skin lesions in Jiangcan Sicao Decoction group were relatively relieved, the degree of skin lesions in Jiangcan Sicao Decoction group was the lowest. In blank group, the skin layers were clear, the tissue structure was not abnormal, and the epidermal thickness was normal; the skin lesions of model group showed psoriasis like changes, and the degree of keratosis of epidermal cells was reduced in the skin lesions of the mice in the Jiangcan Sicao Decoction group. Serum levels of IL-17A, IL-23, TNF-α, TGF-β, and TWEAK in model group were higher than those in blank group (P＜0.05); the serum levels of IL-17A, IL-23, TNF-α, TGF-β, and TWEAK in Jiangcan Sicao Decoction high-concentration, medium-concentration, and low-concentration groups were lower than those in model group (P＜0.05); the serum levels of IL-17A, IL-23, TNF-α, TGF-β, and TWEAK of mice in Jiangcan Sicao Decoction medium-concentration group were lower than those in Jiangcan Sicao Decoction low-concentration, the serum levels of IL-17A, IL-23, TNF-α, TGF-β, and TWEAK in Jiangcan Sicao Decoction high-concentration group were higher than those in Sicao Decoction medium-concentration group (P＜0.05). Conclusion Jiangcan Sicao Decoction can improve the skin lesions of psoriasis mice model, inhibit the secretion of IL-17A, IL-23, TNF-α, and TGF-β, down-regulate the expression of TWEAK. It may play a therapeutic role by down-regulating TWEAK/Fn14 signal, but the specific mechanism needs further study.

Key words： Psoriasis Jiangcan Sicao Decoction Mice Mechanism

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	DUAN Lina1 REN Yu1 JING Huiling2 WANG Xingxing2 WANG Weiying3

Cite this article:

DUAN Lina1 REN Yu1 JING Huiling2 WANG Xingxing2 WANG Weiying3. Study on the therapeutic effect and mechanism of Jiangcan Sicao Decoction on imiquimod-induced psoriasis mice model[J]. 中国医药导报, 2023, 20(6): 28-32.

URL:

https://www.yiyaodaobao.com.cn/EN/10.20047/j.issn1673-7210.2023.06.06 OR https://www.yiyaodaobao.com.cn/EN/Y2023/V20/I6/28

[1] 袁佳晴，朱新景，王星星，等.基于“气血两清”理论探讨浮萍-紫草对银屑病模型小鼠皮肤屏障功能的影响[J].广州中医药大学学报，2022，39（2）：395-401.
[2] Blauvelt A. Safety of secukinumab in the treatment of psoriasis [J]. Expert Opin Drug Saf，2016，15（10）：1413-1420.
[3] 王绾江，王维英，张凡，等.王维英治疗过敏性紫癜的临床经验浅析[J].世界中医药，2020，15（17）：2625-2629.
[4] Chen C，Hou G，Zeng C，et al. Metabolomic profiling reveals amino acid and carnitine alterations as metabolic signatures in psoriasis [J]. Theranostics，2021，11（2）：754-767.
[5] Bocheńska K，Smolińska E，Moskot M，et al. Models in the Research Process of Psoriasis [J]. Int J Mol Sci，2017，18（12）：2514.
[6] Nakajima K，Sano S. Mouse models of psoriasis and their relevance [J]. J Dermatol，2018，45（3）：252-263.
[7] 曹爽，周妍妍，闫景东.中医药调控银屑病相关信号通路研究进展[J].中国实验方剂学杂志，2021，27（15）：243- 250.
[8] 任静，夏育民.TWEAK/Fn14信号在银屑病发病机制中的作用及潜在诊疗策略[J].中国皮肤性病学杂志，2021，35（2）：212-216.
[9] 魏春阳，齐国先，王衍富，等.TWEAK及Fn14在衰老大鼠血管平滑肌细胞中的表达及意义[J].大连医科大学学报，2021，43（3）：198-203，223.
[10] 匡薇薇，魏志平，任虹.窄谱中波紫外线对寻常型银屑病患者外周血Th17细胞及血清IL-17、IL-22、IL-23表达的影响[J].标记免疫分析与临床，2018，25（11）：1627- 1629，1633.
[11] 赵莎，潘敏，陈菲菲，等.紫草素对银屑病样动物模型Th17细胞相关因子的影响[J].临床皮肤科杂志，2016， 45（5）：323-327.
[12] Wang H，Wang S，Li L，et al. Involvement of the cytokine TWEAK in the pathogenesis of psoriasis vulgaris，pustular psoriasis，and erythrodermic psoriasis [J]. Cytokine，2021， 138：155391.
[13] 中国中医科学院广安门医院.朱仁康临床经验集[M].北京：人民卫生出版社，1979：153-158.
[14] 瞿幸.中医皮肤性病学[M].北京：中国中医药出版社，2019.
[15] 杨珂，李靖，何俊伯，等.风邪于IgA肾病的影响[J].中国中西医结合肾病杂志，2021，22（1）：77-79.
[16] 杨素清，柏青松，王姗姗，等.王玉玺运用解表法治疗银屑病经验[J].辽宁中医药大学学报，2018，20（12）：9-11.
[17] 杨素清，张琼，王姗姗.应用虫类药治疗银屑病的经验总结[J].四川中医，2017，35（8）：32-34.
[18] 吕景山.施今墨对药[M].北京：人民军医出版社，2010：55-57.
[19] 史丽颖，丁辉，卢轩，等.槲皮素抗大鼠速发型过敏反应[J].中成药，2016，38（9）：1906-1909.
[20] 胡营杰，孙凤平，韩雪.蝉蜕、僵蚕药对在小儿常见疾病中的应用[J].中国中西医结合儿科学，2020，12（4）：295- 297.
[21] 张驰，杨届.蝉蜕的药理作用及临床应用研究进展[J].湖南中医杂志，2014，30（11）：194-195.
[22] 张凡，王星星，朱新景，等.紫草素通过调控Hippo-YAP信号通路对咪喹莫特诱导银屑病小鼠保护作用的研究[J].现代中西医结合杂志，2021，30（6）：578-583.
[23] 王洁，赵菊花，李达，等.紫草素调控TGF-β1/Survivin通路对银屑病皮肤炎症反应的影响[J].四川中医，2021， 39（10）：49-53.
[24] 韩盈盈，徐曦，王景乐，等.紫草素联合当归饮子方调控IL-23/IL-17轴对咪喹莫特诱导银屑病样小鼠模型的保护作用[J].中国皮肤性病学杂志，2022，36（10）：1137- 1143，1171.
[25] 何俊，樊瑜琪，杨丰文，等.马鞭草化学成分及药理活性研究进展[J].天津中医药，2020，37（11）：1205-1212.
[26] 李海峰，肖凌云，张菊，等.茜草化学成分及其药理作用研究进展[J].中药材，2016，39（6）：1433-1436.