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| Study on the protective mechanism of Bakuchiol against Streptozotocin induced diabetic cardiomyopathy in rats |
| KANG Yongjian1 ZHENG Quanyou2 WANG Xi3▲ |
1.Department of Anesthesiology, 958 Hospital of PLA Army, Chongqing 400000, China;
2.Department of Urology, 958 Hospital of PLA Army, Chongqing 400000, China;
3.Department of Respiratory Medicine, 958 Hospital of PLA Army, Chongqing 400000, China |
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Abstract Objective To investigate the protective mechanism of Bakuchiol against Streptozotocin induced diabetic cardiomyopathy in rats. Methods Forty-five 8-week-old SPF male SD rats, weighing 180-220 g, were randomly divided into control group, diabetes mellitus group, and Bakuchiol group, with 15 rats in each group. After four weeks of high sugar and fat diet (HFD), the diabetes mellitus group and Bakuchiol group were injected intraperitoneal with Streptozotocin (50 mg/kg) every day. After four weeks of normal diet, the control group was injected intraperitoneally with the same amount of normal saline every day, all the three groups were injected continuously for three days. One week after intraperitoneal injection of Streptozotocin, the three groups were orally given glucose (2 g/kg) on an empty stomach for 12 h, and blood glucose values were recorded at 0, 30, 60, 90, and 120 min. The diabetes mellitus group and Bakuchiol group were considered to be successful in modeling with random blood glucose ≥16.7 mmol/L, the two groups continued to be fed HFD for eight weeks, and the Bakuchiol group was also given Bakuchiol (60 mg/kg) gavage for eight weeks. The control group continued the normal diet for eight weeks. After the intervention, the cardiac function was detected by small animals ultrasound, the heart tissue was taken for hematoxylin-eosin staining and Masson staining, the myocardial apoptosis index was detected by TUNEL staining, and the expression level of myocardial apoptosis related proteins was detected by Western blotting. Results Hematoxylin-eosin staining and Masson staining showed that the myocardial arrangement was disordered in the diabetes mellitus group and the perivascular collagen fibers increased significantly, while the myocardial disorder was improved in the Bakuchiol group and the perivascular collagen fibers decreased significantly. Left ventricular ejection fraction, left ventricular short axis shortening rate, and Bcl-2 levels in diabetes mellitus group were lower than those in control group, while the area under the blood glucose curve, myocardial apoptosis index, Cleaved caspase-3/caspase-3, and Bax expression level in model group were higher than those in control group, the differences were highly statistically significant (P＜0.01). Left ventricular ejection fraction, left ventricular short axis shortening rate in Bakuchiol group were higher than those in diabetes mellitus group, while the area under the blood glucose curve, myocardial apoptosis index, Cleaved caspase-3/caspase-3, Bcl-2, and Bax expression level in Bakuchiol group were lower than those in diabetes mellitus group, the differences were statistically significant (P＜0.05 or P＜0.01). Conclusion Bakuchiol treatment can Significantly reduce streptozotocin induced diabetic cardiomyopathy in rats, inhibit apoptosis signal, reduce myocardial cell apoptosis, and improve cardiac function.
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