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| Study on the therapeutic effect and mechanism of Pingxuan Formula on cervical spondylotic arteriopathy model SD rats |
| WANG Yixuan1 PENG Nan2 XIAO Jingjing1 LI Tenglong1▲ LI Lu1 |
1.The First Department of Orthopedics, the Second Affiliated Hospital of Hunan University of Chinese Medicine, Hunan Province, Changsha 410005, China; 2.Department of Orthopaedics and Traumatology Traditional Chinese Medicine, the First People’s Hospital of Shuangliu District, Sichuan Province, Chengdu 610200, China
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Abstract Objective To investigate the effects of Pingxuan Formula on hemorheology, plasma endothelin (ET), and serum nitric oxide (NO) expression levels, and the medial membrane thickness of the vertebral artery wall in the rat model of vertebral arterial cervical spondylosis. Methods According to random number table method, 60 SPF SD rats were divided into normal group, model group, Flunarizine group, Pingxuan Formula low, medium, and high dose groups, with ten rats in each group. In addition to the normal group, the rats with vertebral artery type cervical spondylosis were established by modified hardener molding method. After four weeks of modeling, the Pingxun Formula low, medium, and high dose groups were given oral administration of concentrated liquid of Pingxun Formula 7.8, 15.6 and 31.2 g/kg, respectively, and the Fluunarizine group was given oral administration of Fluunarizine Hydrochloride Capsules 0.9 mg/kg, respectively. The normal group and model group were not treated. After two weeks of drug treatment, the blood flow of the vertebral artery, hemorheology indexes (whole blood viscosity, plasma viscosity, whole blood low/high shear relative index, red blood cell aggregation index), plasma ET, and serum NO levels, and the medial membrane thickness of the vertebral artery wall were measured in each group. Results Compared with the normal group, the average blood flow velocity of the vertebral artery in the model group was decreased, the pulsation index and resistance index of vertebral were increased, and the indexes of hemorheology were increased, the plasma ET content was increased, the plasma NO content was decreased, and the middle membrane thickness of the vertebral artery wall was increased, with statistical significance (P＜0.05). Compared with model group, the average blood flow velocity, pulse index and resistance index of vertebral artery were increased, the indexes of hemorheology were decreased, the plasma ET content was decreased, the plasma NO content was increased, and the middle membrane thickness of vertebral artery wall was decreased, with statistical significances (P＜0.05). Conclusion Pingxuan Formula effectively improve the vertebral artery blood flow and blood viscosity, relieve vasospasm and inhibit the proliferation of vascular smooth muscle cells in the model rats with cervical spondylotic arteriopathy, which maybe one of the mechanisms of Pingxuan Formula in treating cervical spondylotic arteriopathy in rats.
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