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| Optimization of extraction process and antioxidant activity of total phenol from Rhodiola crenulata（HooK. f. et.Thoms）H. Ohba |
| SUN Yuke1 XIE Yelin1 TANG Xiaohui2 ZHAO Wenji2 HE Zhengjun2 JIA Guofu2 LI Jian3 CHEN Yuhang1 |
1.School of Pharmacy, Chengdu Medical College, Sichuan Province, Chengdu 610500, China;
2.Research Center of Engineering Technology of Ecological Remediation of Alpine Grassland in Qinghai-Tibet Plateau, Sichuan Academy of Grassland Sciences, Sichuan Province, Chengdu 611731, China;
3.Institute of Agro-Products Processing, Sichuan Academy of Agricultural Sciences, Sichuan Province, Chengdu 610066, China
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Abstract Objective To optimize the extraction process of total phenol from Rhodiola crenulata（HooK. f. et.Thoms）H. Ohba by response surface method, and to analyze the content of total phenol and antioxidant activity of Rhodiola crenulata（HooK. f. et.Thoms）H. Ohba and their correlation, so as to provide scientific basis for resource exploitation and appropriate harvesting time of Rhodiola crenulata（HooK. f. et.Thoms）H. Ohba. Methods On the basis of single factor test, Box-Behnken response surface method was used to optimize the extraction process of total phenol from Rhodiola crenulata（HooK. f. et.Thoms）H. Ohba. The effects of Rhodiola crenulata（HooK. f. et.Thoms）H. Ohba extract on the scavenging ability of DPPH· and ABTS+· were determined. Results The optimum extraction conditions of Rhodiola crenulata（HooK. f. et.Thoms）H. Ohba were as follows: ethanol concentration of 50%, extraction temperature of 50℃, and extraction time of 40 min. Under these conditions, the extraction rate could reach 12.22 mg/g. The total phenol content and antioxidant activity of wild samples were significantly higher than those of artificial cultivars, and the total phenol content of artificial cultivars was the highest on September 20. The DPPH· scavenging ability of Rhodiola crenulata（HooK. f. et.Thoms）H. Ohba extract was stronger than ABTS+· scavenging ability. The total phenol content was negatively correlated with median inhibitory concentration(IC50) values of DPPH· and ABTS+·（r＜0, P＜0.01）. Conclusion The theoretical values of the response surface optimization method are consistent with the measured values, which can be used to extract and predict the total phenol of Rhodiola crenulata（HooK. f. et.Thoms）H. Ohba. Rhodiola crenulata（HooK. f. et.Thoms）H. Ohba have strong antioxidant activity in vitro and is positively correlated with total phenol content. The suitable harvest time of Rhodiola crenulata（HooK. f. et.Thoms）H. Ohba artificial cultivars is late September
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