Diagnosis value of serum leukocyte immunoglobulin-like receptor subfamily B2 level in the diagnosis of colorectal cancer
GENG Yan1 HUANG Yan2 WANG Zichuan1 LIU Tianzi1 PENG Xintong1 LIU Jing2
1.School of Clinical Medicine, Weifang Medical University, Shandong Province, Weifang 261053, China;
2.Department of Oncology, Affiliated Hospital of Weifang Medical University, Shandong Province, Weifang 261041, China
Abstract Objective To investigate the diagnosis value of leukocyte immunoglobulin-like receptor subfamily B2 (LILRB2) levels in patients with colorectal cancer (CRC). Methods A total of 125 CRC patients diagnosed in Affiliated Hospital of Weifang Medical University （“our hospital” for short） from January 2021 to May 2022 were selected as CRC group, and 120 patients who underwent physical examination in our hospital during the same period were selected as control group. The serum LILRB2, carcinoembryonic antigen (CEA), and carbohydrate antigen 199 (CA199) levels of the CRC group and control group were detected using enzyme-linked immunosorbent assay and electrochemiluminescence method, respectively. Data were statistically processed to analyze the diagnostic value of LILRB2 in patients with CRC. Results Serum LILRB2, CA199, and CEA expressions in the CRC group were higher than those in the control group, the differences were statistically significant (P＜0.05). Statistically significant differences were observed in serum LILRB2 levels among patients of different ages, lymph node metastasis, vascular tumor thrombus, depths of invasions, and TNM stages (P＜0.05). Receiver operating characteristic curve results showed, the area under curve for serum LILRB2, CA199, CEA, and combined detection of the three were 0.7593, 0.5727, 0.7234, and 0.7889, respectively. The diagnostic efficiency of the combined detection of the three was higher than that of CA199 and CEA alone, the differences were statistically significant(P＜0.05). There were no significant differences between the combined detection of the three and LILRB2 (P＞0.05). The diagnostic sensitivity of LILRB2 in TNM Ⅰ+Ⅱ patients was higher than that in TNM Ⅲ+Ⅳ patients, the differences were statistically significant (P＜0.05). Conclusion Serum LILRB2 level in CRC patients has certain clinical value in diagnosis of CRC.
GENG Yan1 HUANG Yan2 WANG Zichuan1 LIU Tianzi1 PENG Xintong1 LIU Jing2. Diagnosis value of serum leukocyte immunoglobulin-like receptor subfamily B2 level in the diagnosis of colorectal cancer[J]. 中国医药导报, 2023, 20(14): 102-105,127.
[1] Siegel RL，Miller KD，Fuchs HE，et al. Cancer statistics，2022 [J]. CA Cancer J Clin，2022，72（1）：7-33.
[2] Sung H，Ferlay J，Siegel RL，et al. Global Cancer Statistics 2020：GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries [J]. CA Cancer J Clin，2021，71（3）：209-249.
[3] Xia C，Dong X，Li H，et al. Cancer statistics in China and United States，2022：profiles，trends，and determinants [J]. Chin Med J （Engl），2022，135（5）：584-590.
[4] Miller KD，Nogueira L，Mariotto AB，et al. Cancer treatment and survivorship statistics，2019 [J]. CA Cancer J Clin，2019， 69（5）：363-385.
[5] Kang X，Kim J，Deng M，et al. Inhibitory leukocyte immuno- globulin-like receptors： Immune checkpoint proteins and tumor sustaining factors [J]. Cell Cycle，2016，15（1）：25-40.
[6] Mitsunori SH，Kouhei T，Kimie A，et al. Human inhibitory rec- eptors Ig-like transcript 2 （ILT2） [J]. PNAS，2003，100（15）：8856-8861.
[7] Zheng J，Umikawa M，Cui C，et al. Inhibitory receptors bind ANGPTLs and support blood stem cells and leukaemia development [J]. Nature，2012，485（7400）：656-660.
[8] Liu X，Zeng H，Yu X，et al. ANGPTL2/LILRB2 signaling promotes the propagation of lung cancer cells [J]. Oncotarget，2015，6（25）：21004-21015.
[9] Carosella ED，Gregori S，Tronik-Le Roux D. HLA-G/LILRBs：A Cancer Immunotherapy Challenge [J]. Trends Cancer，2021，7（5）：389-392.
[10] Sun Y，Liu J，Gao P，et al. Expression of Ig-like transcript 4 inhibitory receptor in human non-small cell lung cancer [J]. Chest，2008，134（4）：783-788.
[11] Zhang P，Yu S，Li H，et al. ILT4 drives B7-H3 expression via PI3K/AKT/mTOR signalling and ILT4/B7-H3 co-expression correlates with poor prognosis in non-small cell lung cancer [J]. FEBS Lett，2015，589（17）：2248-2256.
[12] Liu J，Wang L，Gao W，et al. Inhibitory receptor immun- oglobulin-like transcript 4 was highly expressed in primary ductal and lobular breast cancer and significantly correlated with IL-10 [J]. Diagnostic Patholog，2014，9（85）：85-92.
[13] Fan J，Han J，Li J，et al. The expression and function of immunoglobulin-like transcript 4 in dendritic cells from patients with hepatocellular carcinoma [J]. Hum Immunol，2020， 81（12）：714-725.
[14] Garcia M，Palma MB，Verine J，et al. The immune-checkpoint HLA-G/ILT4 is involved in the regulation of VEGF expression in clear cell renal cell carcinoma [J]. BMC Cancer，2020，20（1）：624.
[15] Li Q，Li J，Wang S，et al. Overexpressed immunoglobulin-like transcript （ILT） 4 in lung adenocarcinoma is correlated with immunosuppressive T cell subset infiltration and poor patient outcomes [J]. Biomarker Research，2020，8（1）：11.
[16] Yang Z，Gao A，Shi W，et al. ILT4 in Colorectal Cancer Cells Induces Suppressive T Cell Contexture and Disease Progression [J]. Onco Targets Ther，2021，14：4239-4254.
[17] 孙秋月，张翔翔.粪便隐血试验及肿瘤标志物联合检测在筛检结直肠癌中的应用价值[J].人人健康，2022，（17）： 90-92.
[18] 谭松松，杨一晖，顾凯，等.中国不同地区人群结直肠癌筛査结肠镜顺应性的综合性分析[J].中华肿瘤防治杂志，2021，28（17）：1283-1289.
[19] 杜佳，张艳，刘秀，等.2012—2019年重庆市城市居民结直肠癌筛查结果分析[J].中国肿瘤，2022，31（5）：355-360.
[20] Astolfi M，Rispoli G，Anania G，et al. Colorectal Cancer Study with Nanostructured Sensors： Tumor Marker Screening of Patient Biopsies [J]. Nanomaterials，2020，10（4）：606.
[21] Xu W，Zhou G，Wang H，et al. Circulating lncRNA SNHG11 as a novel biomarker for early diagnosis and prognosis of colorectal cancer [J]. Int J Cancer，2020，146（10）：2901-2912.
[22] Uhan S，Zidar N，Toma?觩i■ A，et al. Hypermethylated promo- ters of genes UNC5D and KCNA1 as potential novel diagnostic biomarkers in colorectal cancer [J]. Epigenomics，2020， 12（19）：1677-1688.
[23] 袁仁顺，施海华.肿瘤异常糖链蛋白在结直肠癌诊疗中的应用价值[J].中国肿瘤外科杂志，2022，14（2）：175- 177，181.
[24] 黄晨蕾，王莹莹，刘小岑，等.血清CEACAM1对结直肠癌诊断的临床价值分析[J].皖南医学院学报，2021，40（5）：442-444.
[25] 徐浩，杨杰，袁园.LAMβ1联合ARPC4检测在结直肠癌诊断中的应用[J].中国医药导报，2022，19（20）：100- 103.
[26] 汪淑映，罗飞，鲍瑜.miRNA-1260b、miRNA-182-5p及CEA、CA199表达水平对结直肠癌诊断的价值研究[J].临床和实验医学杂志，2022，21（13）：1393-1397.
[27] 王雪虎，郭海峰，殷小平，等.基于CT影像组学的结直肠癌肝转移与原发性肝癌病灶分类研究[J].北京生物医学工程，2021，40（6）：551-556.
[28] Zhang Y，Zhao J，Qiu L，et al. Co-expression of ILT4/HLA- G in human non-small cell lung cancer correlates with poor prognosis and ILT4-HLA-G interaction activates ERK signaling [J]. Tumour Biol，2016，37（8）：11187-11198.
[29] Cai Z，Wang L，Han Y，et al. Immunoglobulinlike transcript 4 and human leukocyte antigen-G interaction promotes the progression of human colorectal cancer [J]. Int J Oncol，2019， 54（6）：1943-1954.
