Abstract Objective To observe the antiviral action and mechanism of Kujin Tablets by stimulating RAW264.7 with Poly (I∶C) in vitro to simulate virus infection. Methods Cells were divided into control group, Poly (I∶C) group and Kujin Tablets group (1 mg/ml). The Poly (I∶C) group was stimulated with 20 μg/ml of Poly (I∶C) for 12 h, after Poly (I∶C) stimulation, the Kujin Tablets group was treated with the Kujin Tablets (1 mg/ml) for 6 h, while the control group received no special treatment. Antiviral factors (Toll-like receptor 3 [TLR3], interferon regulatory factor-3 [IRF3], interferon-β [IFN-β], interferon stimulated gene 15 [ISG15], oligosadenylate synthase 1[OAS1], myxovirus resistance 1 [MX1]), inflammatory factors (interleukin-6 [IL-6], tumor necrosis factor -α [TNF-α], transforming growth factor -β [TGF-β], endoplasmic reticulum stress gene (inositol-requiring enzyme 1α [IRE1α], spliced X-box binding protein 1 [xBP1s], and C/EBP homologous protein [CHOP]) mRNA levels; and IL-6, TNF-α, xBP1s, and caspase-3 protein levels were detected in all groups. Results The mRNA expression levels of TLR3, IRF3, IFN-β, ISG15, OAS1, MX1, IL-6, TNF-α, TGF-β, IRE1α, xBP1s, and CHOP in Poly (I∶C) group were higher than those in control group, and the differences were statistically significant (P ＜ 0.05). Compared with Poly (I∶C) group, the mRNA expression levels of TLR3, IRF3, IFN-β, ISG15, OAS1, and MX1 in Kujin Tablets group were up-regulated, while the mRNA expression levels of IL-6, TNF-α, TGF-β, IRE1α, xBP1s, and CHOP were down-regulated, and the differences were statistically significant (P ＜ 0.05). The protein expression levels of IL-6, TNF-α, xBP1s, and caspase-3 in Poly (I∶C) group were higher than those in control group, and the differences were statistically significant (P ＜ 0.05). The protein expression levels of IL-6, TNF-α, xBP1s, and caspase-3 in Kujin Tablets group were lower than those in Poly (I∶C) group, and the differences were statistically significant (P ＜ 0.05). Conclusion Kujin Tablets can reduce virus-induced apoptosis, and its mechanism is related to promoting intrinsic antiviral response, reducing the release of inflammatory mediators and regulating endoplasmic reticulum stress.
ZHU Chunxue HE Yanjie HE Meijuan LIU Qing LIU Chengyu WANG Yicheng HUANG Hanpeng▲. Effects of Kujin Tablets on macrophages viral infection stimulated by Poly（I∶C）#br#[J]. 中国医药导报, 2022, 19(3): 13-17.
