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| Effect of sparrow-pecking moxibustion on primary dysmenorrhea rats based on EP2/NLRP3 signal pathway |
| HU Jing WEI Wan GAO Hui XU Gang YANG Huayuan#br# |
College of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
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Abstract Objective To investigate the regulation of prostaglandin E receptor 2 (EP2) / Nod-like receptor protein 3 (NLRP3) and its anti-inflammatory mechanism in primary dysmenorrhea rats by sparrow-pecking moxibustion. Methods A total of 24 female SD rats were divided into blank group, model group, sprrow-pecking moxibustion group, and agonist group, with six rats in each group. Except blank group, the primary dysmenorrhea model was established for the other three groups, blank group received the same dose of normal saline daily. Blank group and model group were not treated; in sparrow-pecking moxibustion group, sānyīnjiāo and gu?觀nyuán acupoints were selected for treatment after daily modeling; agonist group was intraperitoneally injected with Treprostinil daily after modeling, followed by sparrow-pecking moxibustion treatment, which was the same as the sparrow-pecking moxibustion group. The latent time and number of torsion were observed in each group; HE staining was used to observe the pathological conditions of uterus; the expressions of EP2, NLRP3, and caspase-1 proteins were detected by immunohistochemistry. Results Compared with blank group, the latent time was shortened and the number of torsion was increased in model group (P < 0.01); compared with model group, the latent time was prolonged and the number of torsion was decreased in sparrow-pecking moxibustion group (P < 0.01); compared with sparrow-pecking moxibustion group, the latent time was shortened and the number of torsion was increased in agonist group (P < 0.01). In blank group, the uterine tissue morphology was regular, there were a few lymphocytes in the interstitium of muscle cells, and no neutrophil infiltration; in model group and agonist group, small uterine vessels showed dilation and hyperemia, and inflammatory cells such as lymphocytes and eosinophils were infiltrated outwards as focal groups; in sparrow-pecking moxibustion group, the degree of interstitial hyperemia and edema and infiltration of inflammatory cells were less than that in model group, a few inflammatory cells infiltrated between muscle walls and only a few small blood vessels hyperemia were observed. Compared with blank group, the protein expressions of EP2, NLRP3, and caspase-1 were increased in model group (P < 0.01); compared with model group, the protein expressions of EP2, NLRP3, and caspase-1 were decreased in sparrow-pecking group (P < 0.01); compared with sparrow-pecking group, the protein expressions of NLRP3 and caspase-1 were increased in agonist group, while the protein expression of EP2 in sparrow-pecking group and agonist group was not significantly different (P > 0.05). Conclusion Sparrow-pecking moxibustion can improve the infiltration of inflammatory cells in the uterus, and regulate the inflammatory response of dysmenorrhea rats by regulating EP2/NLRP3 signal pathway.
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[1] Ferries-Rowe E，Corey E，Archer JS. Primary Dysmenorrhea：Diagnosis and Therapy [J]. Rev Bras Ginecol Obstet，2020，136（5）：1047-1058.
[2] 林文爱.热敏灸治疗寒凝血瘀型原发性痛经的临床疗效观察[D].福州：福建中医药大学，2021.
[3] 成鸿群，刘宜成，徐金鹏，等.温和灸和雀啄灸温度场分布特点的实验研究[J].中国针灸，2021，41（10）：1113-1117.
[4] 袁琳娜，贾红玲.针灸为主治疗原发性痛经的研究进展[J].湖南中医杂志，2014，30（11）：205-207.
[5] Martinon F，Burns K，Tschopp J. The Inflammasome：A Molecular Platform Triggering Activation of Inflammatory Caspases and Processing of proIL-β [J]. Mol Cell，2002， 10（2）：417-426.
[6] Tang B，Liu D，Chen L，et al. NLRP3 inflammasome inhibitor MCC950 attenuates primary dysmenorrhea in mice via the NF-kB/COX-2/PG pathway [J]. J Inflam，2020，17：22.
[7] Han SJ，Jung SY，Wu SP，et al. Estrogen Receptor β Modulates Apoptosis Complexes and the Inflammasome to Drive the Pathogenesis of Endometriosis [J]. Cell，2015， 163（4）：960-974.
[8] Wang M，Wang Y，Xie T，et al. Prostaglandin E2/EP2 receptor signalling pathway promotes diabetic retinopathy in a rat model of diabetes [J]. Diabetologia，2019，62（2）：335-348.
[9] 朱飞鹏.常用医药研究动物模型[M].北京：人民卫生出版社，2007.
[10] 孙小雪，梁玉磊，李新华，等.近十年针灸干预对原发性痛经动物模型影响的实验研究进展[J].河北中医药学报，2017，32（1）：60-64.
[11] 公春荣.基于CNKI数据库对针灸治疗痛经的取穴规律研究[J].中国医药导报，2021，18（22）：125-128.
[12] Schoep ME，Adang EMM，Maas JWM，et al. Productivity loss due to menstruation-related symptoms：a nationwide cross-sectional survey among 32 748 women [J]. BMJ Open，2019，9（6）：e026186.
[13] 陈鹏典，杨卓欣，宁艳，等.针灸干预原发性痛经机制最近研究进展[J].中国医药导报，2018，15（30）：32-35.
[14] 黄冰晴，王兵丽.前列腺素E2及其受体对炎性痛调制的研究进展[J].闽南师范大学学报（自然科学版），2017， 30（2）：65-70.
[15] Kelly P，Meade KG，O’Farrelly C. Non-canonical Inflammasome-Mediated IL-1β Production by Primary Endometrial Epithelial and Stromal Fibroblast Cells Is NLRP3 and Caspase-4 Dependent [J]. Front Immunol，2019，10：102.
[16] 薛瑞，苗一非，杨吉春，等.前列腺素E2对免疫细胞及炎症相关疾病的调控作用[J].生理科学进展，2011，42（3）：165-168.
[17] 苗芙蕊，陈洁洁，芮靖琳，等.隔药灸命门穴对痛经模型大鼠血清前列腺素水平的影响[J].时珍国医国药，2014， 25（5）：1235-1237.
[18] Ma B，Yang S，Tan T，et al. An integrated study of metabolomics and transcriptomics to reveal the anti-primary dysmenorrhea mechanism of Akebiae Fructus [J]. J Ethnopharmacol，2021，270：113763.
[19] 孙小雪.不同时机艾灸对寒湿凝滞型痛经大鼠子宫组织微血管密度及血管活性物质含量的影响[D].石家庄：河北医科大学，2017.
[20] 潘亚辉，李姝婧，马玉宁，等.脐疗对寒凝血瘀型痛经大鼠扭体反应及神阙穴区TLR4的影响[J].中华中医药杂志，2019，34（8）：3697-3700.
[21] 任晓暄.电针不同穴位对实验性类痛经大鼠镇痛效应及其机理的研究[D].北京：中国中医科学院，2010.
[22] 苏同生，强军，万兆新，等.雀啄灸热源不同频率感受性差异30例[J].陕西中医，2014，35（6）：733-734.
[23] 王乙钦.穴位埋线对原发性痛经大鼠子宫组织核苷酸结合寡聚化结构域受体炎性小体的影响[J].针刺研究，2019，44（7）：481-485.
[24] 唐文静，王乙钦，唐标.穴位埋线对原发性痛经大鼠子宫组织前列腺素相关因子和核转录因子κB的影响[J].针刺研究，2020，45（7）：548-551.
[25] 王曼.PGE2/EP2R信号通路上调NLRP3炎性小体活性促进DR发生发展的作用及机制研究[D].南京：南京医科大学，2019.
[26] 李南，孙妩弋，孙家昌，等.炎症小体在纤维化疾病中作用的研究进展[J].中国药理学通报，2020，36（4）：479-483. |
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