lncRNAs H19在糖尿病骨关节炎软骨下骨中的表达及其意义

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Abstract Objective To investigate the expression of lncRNAs H19 in the subchondral bone of diabetic mellitus osteoarthritis (OA) and its significance. Methods Three patients with diabetes mellitus OA (DM-OA group), three patients with non diabetes OA (OA group) and, meanwhile, three physical examinees of the control group were selected. The expression of H19 in subchondral bone of each group was compared, H19 overexpression plasmid was constructed, and miR-141 and miR-22 mimic analogues were synthesized. The expression of β-catenin protein was detected. The differentiation function of osteoblasts was detected. Results The expression level of H19 in DM-OA group was higher than that in OA group and control group, and the expression levels of miR-141 and miR-22 in DM-OA group were lower than those in OA group and control group (P ＜ 0.05). The expression level of H19 in OA group was higher than that in control group, and the expression levels of miR-141 and miR-22 in OA group were lower than those in control group (P ＜ 0.05). After overexpression of H19 WT, miR-141, and miR-22 fragments, the fluorescence signal reported by luciferase decreased significantly (P ＜ 0.01). The miR-141 mimic and miR-22 mimic analogues had no effect on the fluorescence signal of mutant plasmids, and the difference was not statistically significant (P ＞ 0.05). The expression levels of β-catenin mRNA and Runx2 mRNA in DM-OA group were higher than those in OA group and control group (P ＜ 0.05). The expression levels of β-catenin mRNA and Runx2 mRNA in OA group were higher than those in control group (P ＜ 0.05). The expression levels of β-catenin protein and Runx2 protein in DM-OA group were higher than those in OA group and control group (P ＜ 0.05). The expression levels of β-catenin protein and Runx2 protein in OA group were higher than those in control group (P ＜ 0.05). Conclusion H19 is overexpressed in the subchondral bone of diabetes OA. H19 can activate Wnt/β-catenin signaling pathway by regulating miR-141 and miR-22 promotes subchondral bone hyperplasia and sclerosis.

Key words： Osteoarthritis Subchondral bone Osteoblasts H19

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	XUE Wei1 KANG Shaoying1 GUO Hongsheng1 WANG Peixia1 WANG Zhenxing1 ZHAN Jinhua1 ZHANG Yingmin1 WANG Huajun2

Cite this article:

XUE Wei1 KANG Shaoying1 GUO Hongsheng1 WANG Peixia1 WANG Zhenxing1 ZHAN Jinhua1 ZHANG Yingmin1 WANG Huajun2. Expression of lncRNAs H19 and its significance in subchondral bone of diabetic mellitus osteoarthritis[J]. 中国医药导报, 2022, 19(20): 25-29.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I20/25

[1] Coryell PR，Diekman BO，Loeser RF. Mechanisms and therapeutic implications of cellular senescence in osteoarthritis [J]. Nat Rev Rheumatol，2021，17（1）：47-57.
[2] Coleman MC，Ramakrishnan PS，Brouillette MJ，et al. Injurious loading of articular cartilage compromises chondrocyte respiratory function [J]. Arthritis Rheumatol，2016，68（3）：662-671.
[3] Zheng L，Zhang Z，Sheng P，et al. The role of metabolism in chondrocyte dysfunction and the progression of osteoarthritis [J]. Ageing Res Rev，2021，66：101249.
[4] Liu M，Liu SW，Wang LJ，et al. Burden of diabetes，hyperglycaemia in China from 1990 to 2016：findings 4 from the 1990 to 2016，global burden of disease study [J]. Diabetes Metab，2019，45（3）：286-293.
[5] Schett G，Kleyer A，Perricone C，et al. Diabetes is an independent predictor for severe osteoarthritis：results from a longitudinal cohort study [J]. Diabetes Care，2013，36（2）：403-409.
[6] Plasek LM，Valadkhan S. lncRNAs in T lymphocytes：RNA regulation at the heart of the immune response [J]. Am J Physiol Cell Physiol，2021，320（3）：C415-C427.
[7] Taniue K，Akimitsu N. The functions and unique features of lncRNAs in cancer development and tumorigenesis [J]. Int J Mol Sci，2021，22（2）：632.
[8] Dilmaghani NA，Khoshsirat S，Shanaki-Bavarsad M，et al. The contributory role of long non-coding RNAs （lncRNAs） in head and neck cancers：possible biomarkers and therapeutic targets? [J]. Eur J Pharmacol，2021，900：174053.
[9] Huang Y，Zheng Y，Jia L，et al. Long noncoding RNA H19 promotes osteoblast differentiation via TGF-β1/Smad3/HDAC signaling pathway by deriving miR-675 [J]. Stem Cells，2015，33（12）：3481-3492.
[10] Tan F，Wang D，Yuan Z. The fibroblast-like synoviocyte derived exosomal long non-coding RNA H19 alleviates osteoarthritis progression through the miR-106b-5p/TIMP2 axis [J]. Inflammation，2020，43（4）：1498-1509.
[11] Gao Y，Wu F，Zhou J，et al. The H19/let-7 double-negative feedback loop contributes to glucose metabolism in muscle cells [J]. Nucleic Acids Res，2014，42（22）：13799-13811.
[12] Leone R，de Rosa A，Iudicone P，et al. Pain control and functional improvement in patients treated by autologous conditioned serum after failure of platelet rich plasma treatments in knee osteoarthritis [J]. Transfus Med，2021， 31（5）：357-364.
[13] Lo J，Chan L，Flynn S. A systematic review of the incidence，prevalence，costs，and activity and work limitations of amputation，osteoarthritis，rheumatoid arthritis，back pain，multiple sclerosis，spinal cord injury，stroke，and traumatic brain injury in the United States：a 2019 update [J]. Arch Phys Med Rehabil，2021，102（1）：115-131.
[14] Medina-Porqueres I，Ortega-Castillo M，Muriel-Garcia A. Effectiveness of platelet-rich plasma in the management of hip osteoarthritis：a systematic review and meta-analysis [J]. Clin Rheumatol，2021，40（1）：53-64.
[15] Hugle T，Geurts J. What drives osteoarthritis? — Synovial versus subchondral bone pathology [J]. Rheumatology（Oxford），2017，56（9）：1461-1471.
[16] 杨明义，马尧，许珂，等.软骨下骨硬化在骨关节炎发病机制中的作用研究[J].中华关节外科杂志：电子版，2020， 14（5）：602-607.
[17] Radin EL，Rose RM. Role of subchondral bone in the initiation andprogression of cartilage damage [J]. Clin Orthop Relat Res，1986，213：34-40.
[18] Puenpatom RA，Victor TW. Increased prevalence of metabolic syndrome in individuals with osteoarthritis：an analysis of NHANES Ⅲ data [J]. Postgrad Med，2009， 121（6）：9-20.
[19] Brosnan CA，Voinnet O. The long and the short of noncoding RNAs [J]. Curr Opin Cell Biol，2009，21（3）：416-425.
[20] Lv Z，Xu Q，Yuan Y. A systematic review and meta-analysis of the association between long non-coding RNA polymorphisms and cancer risk [J]. Mutat Res Rev Mutat Res，2017，771：1-14.
[21] Hurst LD，Smith NG. Molecular evolutionary evidence that H19 mRNA is functional [J]. Trends Genet，1999，15（4）：134-135.
[22] Goyal N，Kesharwani D，Datta M. Lnc-ing non-coding RNAs with metabolism and diabetes：roles of lncRNAs [J]. Cell Mol Life Sci，2018，75（10）：1827-1837．
[23] Dykes IM，Emanueli C. Transcriptional and post-transcriptional gene regulation by long non-coding RNA [J]. Genomics Proteomics Bioinformatics，2017，15（3）：177-186．
[24] Liang WC，Fu WM，Wang YB，et al. H19 activates Wnt signaling and promotes osteoblast differentiation by functioning as a competing endogenous RNA [J]. Sci Rep，2016，8（6）：20121.
[25] 王秋霏，顾叶，彭育沁，等.人工假体磨损颗粒作用下Wnt/β-catenin信号通路对成骨细胞的影响[J].中国组织工程研究，2021，25（24）：3894-3901.