Abstract Objective To investigate the effect of triiodothyronine (T3) on proliferation of papillary carcinoma of thyroid cells TPC-1 and its mechanism. Methods TPC-1 cells were divided into control group (0 ng/ml T3 treatment group), 12.5 ng/ml T3 treatment group and 50 ng/ml T3 treatment group, and treated with corresponding T3 concentration, respectively. Real-time unlabeled cell function analysis technique (RTCA) and clonal formation assay were used to detect the proliferation ability of the cells, and Western blot was used to detect the expression level of PDZK1 in the cells. TPC-1 cells were divided into knockout PDZK1 (shPDZK1) group, knockout control (shNC) group, overexpression PDZK1 (PDZK1) group and overexpression control (Vector) group by lentivirus transfection method. The expression level of PDZK1 in TPC-1 cells was detected by Western blot. Meanwhile, cells in each group were treated with different concentrations of T3 (0, 12.5, 50 ng/ml), and the clonal formation ability of cells was detected by clonal formation assay. Results The cell index and clone formation number of TPC-1 in 12.5 and 50 ng/ml T3 treatment groups were significantly higher than those in control group, and the differences were highly statistically significant (P ＜ 0.01). The expression levels of PDZK1 in 12.5 and 50 ng/ml T3 treatment groups were significantly higher than that in control group, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). Compared with shNC group, the expression level of PDZK1 in shPDZK1 group was significantly lower, and the difference was statistically significant (P ＜ 0.05). Compared with the Vector group, the expression level of PDZK1 in the PDZK1 group was significantly increased, and the difference was highly statistically significant (P ＜ 0.01). Compared with the shNC group, the clore formation number in the shPDZK1 group was significantly decreased after T3 treatment of 0, 12.5, and 50 ng/ml, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). Compared with Vector group, the clone formation number in PDZK1 group was significantly increased in 0, 12.5, and 50 ng/ml T3 treatments, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). Conclusion T3 may regulate TPC-1 cell proliferation by up-regulating PDZK1 expression.
CHENG Yanan WANG Yan SUN Rongxin LANG Jianan CAO Bin YANG Longyan ZHAO Dong. Effect of T3 on proliferation of papillary carcinoma of thyroid cells TPC-1 and its mechanism#br#[J]. 中国医药导报, 2022, 19(12): 8-12.
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