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| Protective effects of flavonoid of Fructus Kochiae on Paracetamol-induced acute liver injury in mice#br# |
| ZHENG Yi LI Hui WU Jin WANG Fang WU Jia CAI Pei |
| Department of Pharmacy, Hunan Provincial Maternal and Child Health Care Hospital, Hunan Province, Changsha 410008, China |
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Abstract Objective To observe the protective effects of flavonoid of Fructus Kochiae (FFK) on Paracetamol-induced acute liver injury in mice. Methods Thirty-six one-month old SPF Kunming mice with body weight of (20±2) g were selected, male and female half, they were divided into FFK low-dose, medium-dose, and high-dose groups (75, 150, 300 mg/kg) and positive control group (Bifendate, 150 mg/kg), blank control group (equal amount of normal saline), and Paracetamol model group (equal amount of normal saline), with six mice in each group. After seven days of continuous intragastric administration, the blank control group was intraperitoneally injected with normal saline (0.1 ml/10 g), and the other groups were intraperitoneally injected with Paracetamol (350 mg/kg) to establish acute liver injury model. After 24 hours, glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) levels in serum and the contents of lipid peroxides malondialdehyde (MDA) and superoxide dismutase (SOD) in liver tissues were detected, and the histopathological changes of liver tissues were observed by hematoxylin-eosin (HE) staining. Results The GPT, GOT, and MDA in Paracetamol model group were higher than those in blank control group, while SOD was lower than blank control group, the differences were highly statistically significant (P ＜ 0.01). There was no significant difference between FFK low-dose group and Paracetamol model group (P ＞ 0.05). The GPT, GOT and MDA in medium-dose and high-dose FFK groups were lower than those in Paracetamol model group and low-dose FFK group, while SOD was higher than those in Paracetamol model group and low-dose FFK group, with statistical significance (P ＜ 0.05). HE staining results of pathological sections showed different degrees of liver injury in all groups except blank control group. Conclusion A certain does of FFK has a good protective effect on Paracetamol-induced acute liver injury in mice, and its ability to enhance SOD activity in liver tissue and reduce MDA accumulation may be one of the mechanisms of its liver-protecting and lowering enzymes.
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