Effects of ginsenoside Rg1 on viability and vascular differentiation of human umbilical blood mesenchymal stem cells#br#
ZHOU Qimiao1 PENG Ajian2 ZHANG Xi3 ZHU Furong1 TAN Meixin2 WANG Xiaoqin2 PENG Jiarui2 XIONG Wu1
1.Department of Burn Orthopaedics, the First Affiliated Hospital of Hunan University of Chinese Medicine, Hunan Province, Changsha 410007, China; 2.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Hunan Province, Changsha 410208, China; 3.Clinical Medical School, Hunan University of Chinese Medicine, Hunan Province, Changsha 410007, China
Abstract Objective To investigate the effects of ginsenoside Rg1 (GS-Rg1) on the activity and endothelial differentiation of human umbilical cord blood mesenchymal stem cell (MSC) in vitro. Methods Human umbilical blood was collected from normal cesarean placenta under aseptic conditions, and mononuclear cells were isolated by density gradient centrifugation. When the cells were fusiform, osteogenesis, chondrogenesis, and adipogenic differentiation were identified. The identified human umbilical blood MSCs were treated with 0, 5, 10, 20, 40, and 80 mg/L GS-Rg1, respectively, to determine the optimal concentration of GS-Rg1 to promote the proliferation of human umbilical blood MSCs. Then human umbilical blood MSCs were divided into experimental group and control group. The experimental group was treated with optimal concentration of GS-Rg1, and the control group was treated with equal volume of phosphate buffer. CCK-8 method was used to study the cell activity of the two groups, and Matrigel experiment was used to study in vitro angiogenesis of the two groups. Meanwhile, the expression of platelet-endothelial cell adhesion molecule-1 (CD31) and von Willebrand factor (vWF) were detected by immunofluorescence. Results Microscopically, the morphology of the cells was uniform, showing a long spindle fibroblast-like morphology, which was typical of MSCs. The staining results of osteogenic, chondrogenic, and adipogenic differentiation showed a large number of deep staining parts, indicating that they were MSCs. When GS-Rg1 concentration was 40 and 80 mg/L, the MSCs optical density value was higher than that when GS-Rg1 concentration were 0, 5, 10, and 20 mg/L, the differences were statistically significant (P ＜ 0.05). When GS-Rg1 concentration was 80 mg/L, there was no significant difference in the optical density of MSCs when GS-Rg1 concentration was 40 mg/L (P ＞ 0.05). The cell activity, lumen length, and fluorescence intensity of CD31 and vWF in experimental group were higher than those in control group, and the differences were statistically significant (P ＜ 0.05). Conclusion GS-Rg1 has the potential to promote the proliferation of human umbilical blood MSCs in vitro and induce their differentiation into endothelial cells and angiogenesis in vitro.
