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| Intervention of cryptotanshinone on depression induced by chronic corticosterone injection in mice#br# |
| CHEN Mingzhu LIAO Wanting HUANG Youxia |
| School of Pharmacy, Quanzhou Medical College, Fujian Province, Quanzhou 362011, China |
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Abstract Objective To observe the intervention of cryptotanshinone on depression induced by chronic corticosterone (CORT) injection in mice. Methods Sixty male SPF KM mice were selected. According to random number table method, they were divided into cryptotanshinone low-dose group (10 mg/kg), cryptotanshinone medium-dose group (20 mg/kg), cryptotanshinone high-dose group (40 mg/kg), Fluoxetine group (20 mg/kg), normal control group (equal amount of normal saline), and model group (equal amount of normal saline), with ten mice in each group. Except for normal control group, the depression model was established by continuous injection of CORT (20 mg/kg) in other groups, and the model was established at the same time. After 21 days of continuous administration, the effects of cryptotanshinone on body weight gain, sugar water consumption ratio of mice were observed. Behavioral evaluation was conducted by forced swimming test, open field test and water maze test. Serum levels of CORT, adrenocorticotropic hormone (ACTH), and brain-derived neurotrophic factor (BDNF) in hippocampus and prefrontal cortex were determined. Results In model group, the weight gain, sugar water consumption ratio, and BDNF content were lower than those in normal control group, the immobility time, central lattice residence time, and escape latency were longer than those of the normal control group, the number of horizontal crossing lattice, upright times, and modification times were less than those of normal control group, the stay time of the target quadrant and the movement distance around the platform were shorter than those of the normal control group, the contents of CORT and ACTH were higher than those of normal control group, and the differences were statistically significant (P < 0.05 or P ＜ 0.01). In cryptotanshinone dose groups, the weight gain was higher than that of the model group, the central lattice residence time was shorter than that of model group, the number of horizontal crossing lattice and modification times were more than those of model group, and the levels of CORT and ACTH were lower than that of model group, the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). In cryptotanshinone medium-dose and high-dose groups, the sugar water consumption ratio was higher than that of model group, the immobility time was shorter than the model group, the center lattice residence time was shorter than the cryptotanshinone low-dose group, and the upright times were more than the model group and the cryptotanshinone low-dose group, the number of horizontal crossing lattice and the modifications times were more than those of cryptotanshinone low-dose group, the escape latency was shorter than that of the model group, the stay time of the target quadrant and the movement distance around the platform were longer than those of the model group, levels of CORT and ACTH were lower than those of cryptotanshinone low-dose group, and the content of BDNF was higher than that of the model group and the cryptotanshinone low-dose group, with statistical significances (P ＜ 0.05 or P ＜ 0.01). The escape latency in the cryptotanshinone high-dose group was shorter than that in the cryptotanshinone low and medium dose groups, the level of CORT was lower than that in the cryptotanshinone medium-dose group, and the content of BDNF in hippocampus was higher than that in the cryptotanshinone medium-dose group, with statistical significances (P ＜ 0.05 or P ＜ 0.01). Conclusion Cryptotanshinone can improve the depression-like behavior of CORT-induced mice, and the effect is more obvious in the medium-dose and high-dose groups. The mechanism may be related to the regulation of hypothalamic-pituitary-adrenal axis function and the up-regulation of BDNF expression level in brain.
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