Discussion on mechanism of Zhuanyao Decoction in the treatment of degenerative lumbar spinal stenosis based on network pharmacology
LI Luguang1 ZHU Liguo1 GAO Jinghua1，2 GAO Chunyu1 WANG Baojian1 WEI Xu1 YIN Xunlu1 LI Hui1
1.The Second Department of Spine, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China;
2.Beijing Key Laboratory of Orthopaedic Technology of Traditional Chinese Medicine, Beijing 100102, China
Abstract Objective To explore the mechanism of Zhuanyao Decoction in the treatment of degenerative lumbar spinal stenosis (DLSS) based on network pharmacology. Methods The main active components and targets of Zhuanyao Decoction were obtained through TCMSP, BATMAN database and literature, and the “active ingredient-target” network of Zhuanyao Decoction was constructed by Cytoscape. The pathogenic target of DLSS was obtained by DisGeNET, GeneCards and OMIM disease database. The analysis of protein-protein interaction (PPI) was carried out on the String platform. The PPI network was constructed by Cytoscape and the main targets in the network were screened out according to the degree value. Metascape was used to analyze the biological process and pathway of Zhuanyao Decoction in the treatment of DLSS. Results Eighty-six main active components and corresponding 185 targets of Zhuanyao Decoction were screened, 1168 pathogenic targets of DLSS and 84 intersection targets of DLSS treated with Zhuanyao Decoction were screened. PPI showed that it was closely related to JUN, TNF, AKT1, MAPK1, and other targets. There were 1987 biological function items and 142 signal pathways obtained by GO biological function and KEGG pathway analysis. Conclusion Zhuanyao Decoction has the characteristics of multi-component, multi-target and multi-pathway in the treatment of DLSS.
LI Luguang1 ZHU Liguo1 GAO Jinghua1,2 GAO Chunyu1 WANG Baojian1 WEI Xu1 YIN Xunlu1 LI Hui1. Discussion on mechanism of Zhuanyao Decoction in the treatment of degenerative lumbar spinal stenosis based on network pharmacology[J]. 中国医药导报, 2021, 18(13): 13-18.
[1] Kunlong M，Chunfeng C，Qunbo W，et al. The reduction in blood loss with an intravenous drip of tranexamic acid in decompression and fusion surgery for degenerative lumbar spinal stenosis：a randomized controlled trial [J]. Int J Clin Exp Med，2019，12（5）：6116-6121.
[2] 薛志兴，周建伟，张建中，等.骨水泥强化椎弓根钉内固定在老年退行性腰椎管狭窄症手术治疗中的应用[J].中国骨与关节损伤杂志，2019，34（1）：77-79.
[3] Parai C，H?覿gg O，Lind B，et al. Follow-up of degenerative lumbar spine surgery-PROMs stabilize after 1 year：an equivalence study based on Swespine data [J]. Eur Spine J，2019，28（9）：2187-2197.
[4] 高景华，王宝剑，孟州令，等.基于“阳化气”理论探讨转腰汤治疗腰椎管狭窄症48例[J].中国中医骨伤科杂志，2019，27（11）：55-57，61.
[5] Ru J，Li P，Wang J，et al. TCMSP：a database of systems pharmacology for drug discovery from herbal medicines [J]. J Cheminform，2014，6（1）：13.
[6] Liu Z，Guo F，Wang Y，et al. BATMAN-TCM：a bioinformatics analysis tool for molecular mechANism of traditional Chinese medicine [J]. Sci Rep，2016，6：21146.
[7] 牟海栋，屠鹏飞，姜勇.基于网络药理学的肉桂温经通脉的作用机制研究[J].药学学报，2016，51（11）：1724-1733.
[8] 陈晓萍，张长林.白术不同化学成分的药理作用研究概况[J].中医药信息，2011，28（2）：124-126.
[9] 汪小莉，刘晓，夏春燕，等.防己黄芪汤药理作用及各单味药化学成分研究进展[J].中草药，2016，47（19）：3527-3534.
[10] 高春雨，李路广，孟州令，等.高景华运用转腰汤加减治疗退行性脊柱疾病经验[J].中医药导报，2020，26（9）：184-186，197.
[11] 孙淑君，向阳，黄世林.单味中药及其有效成分抗纤维化机制的研究进展[J].中国中药杂志，2008，33（24）：2882-2886.
[12] Park JB，Lee JK，Park SJ，et al. Hypertrophy of ligamentum flavum in lumbar spinal stenosis associated with increased proteinase inhibitor concentration [J]. J Bone Joint Surg Am，2005，87（12）：2750-2757.
[13] Ren J，Li J，Liu X，et al. Quercetin inhibits fibroblast activation and kidney fibrosis involving the suppression of mammalian target of rapamycin and β-catenin signaling [J]. Sci Rep，2016，6：23968.
[14] Cheng D，Guo Z，Zhang S. Effect of β-sitosterol on the expression of HPV E6 and p53 in cervical carcinoma cells [J]. Contemp Oncol（Pozn），2015，19（1）：36-42.
[15] Oh IS，Suh DW，Ha KY. Hypertrophy of the ligament flavum in degenerative lumbar stenosis associated with the increased expression of fractalkine（CX3CL1）/CX3CR1 chemokine [J]. Connect Tissue Res，2013，54（6）：380-385.
[16] Lian JJ，Cheng BF，Gao YX，et al. Protective effect of kaempferol，a flavonoid widely present in varieties of edible plants，on IL-1β-induced inflammatory response via inhibiting MAPK，Akt，and NF-κB signalling in SW982 cells [J]. J Funct Foods，2016，27：214-222.
[17] Nakama S，Kikuchi M，Yashiro T，et al. Regional difference in the appearance of apoptotic cell death in the ligamentum flavum of the human cervical spine [J]. Med Mol Morphol，2005，38（3）：173-180.
[18] Chen YR，Wang X，Templeton D，et al. The role of cJun N-terminal kinase（JNK）in apoptosis induced by ultraviolet C and gamma radiation：Duration of JNK activation may determine cell death and proliferation [J]. J Biol Chemj，1996，271（50）：31929-31936.
[19] 李强，赵建民，张元智，等.腰椎退变黄韧带中炎性细胞因子的表达[J].中国脊柱脊髓杂志，2011，21（1）：63-66.
[20] 黄秀昆，孙雪梅，韦秀桂，等.老鼠簕生物碱A对肝纤维化大鼠PI3K/Akt/m TOR/p70S6K信号通路的影响[J].中草药，2019，50（2）：457-461.
[21] 卢昌怀，刘志军，张宏波，等.p38丝裂原活化蛋白激酶通路介导TGF-β1/结缔组织生长因子调控人腰椎黄韧带增生肥厚的机制研究[J].中国修复重建外科杂志，2019，33（6）：730-735.
[22] Liao R，Sun J，Wu H，et al. High expression of IL-17 and IL-17RE associate with poor prognosis of hepatocellular carcinoma [J]. J Exp Clin Canc Res，2013，32（1）：3.
[23] L?觟hr M，Hampl JA，Lee JY，et al. Hypertrophy of the lumbar ligamentum flavum is associated with inflammation-related TGF-β expression [J]. Acta Neurochir，2011，153（1）：134-141.
[24] Wei L，Liu M，Xiong H，et al. Up-regulation of IL-23 expression in human dental pulp fibroblasts by IL-17 via activation of the NF-κB and MAPK pathways [J]. Int Endod J，2018，51（6）：622-631.
[25] 王源，李万成.HIF-1α与肺纤维化关系的研究进展[J].临床误诊误治，2019，32（10）：109-113.
[26] Nayak BK，Shanmugasundaram K，Friedrichs WE，et al. HIF-1 Mediates Renal Fibrosis in OVE26 Type 1 Diabetic Mice [J]. Diabetes，2016，65（5）：1387-1397.
[27] 伍振辉，孟娴，胡佳伟，等.TLR4-MyD88-NF-κB信号通路与肝炎-肝纤维化-肝癌轴相关性研究进展[J].国际药学研究杂志，2017，44（5）：396-401.
