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| Effect of Shenfu Injection on immunoregulation and inflammatory factors in septic mice |
| ZHAO Fengli1 XIAN Shaoxiang2 LUO Yuanyuan1 WANG Yixin3 XU Yunsheng1 LIN Xinfeng1 |
| 1.Department of Critical Care Medicine, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510405, China; 2.Department of Cardiology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510405, China; 3.Department of Acupuncture, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510405, China |
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Abstract Objective To investigate the effects of Shenfu Injection on immuno regulation and inflammatory factors in septic mice. Methods A total of 120 10-week-old SPF male C57BL/6 mice were selected and divided into control group, sham operation group, septic model group and Shenfu Injection treatment group according to random number table method, with 30 rats in each group. A septic mice model was prepared by cecal ligation and perforation. The control group, sham-operated group and sepsis model group were given intraperitoneal injection of saline [4 mL/(kg·BW) for 3 days] respectively on the first day after operation, and Shenfu Injection group was given intraperitoneal injection of Shenfu Injection [4 mL/(kg·BW) for 3 days] at the same time. T lymphocyte subsets in each group were detected by enzyme-linked immunosorbent assay, hematoxylin-eosin staining was used to evaluate organ pathological damage and inflammatory cell infiltration. Results Compared with the control group, the levels of interleukin-4 (IL-4), IL-17, interferon γ (IFN-γ), IL-10 and IFN-γ/IL-4, IL-10/IL-17 in sham operation group had no significant difference (P > 0.05). Compared with sham-operated group, the levels of IFN-γ and IL-10 in sepsis model group were significantly decreased, IL-4 and IL-17 were significantly increased, and IFN-γ/IL-4 and IL-10/IL-17 were significantly decreased, the differences were statistically significant (P < 0.05), and its pathological damage of heart, lung and kidney was serious, and the infiltration of inflammatory cells was increased (P < 0.05). Compared with sepsis model group, the levels of IFN-γ and IL-10 increased, IL-4 and IL-17 decreased, IFN-γ/IL-4 and IL-10/IL-17 increased in Shenfu injection treatment group, but were lower than those in control group, the difference were statistically significant (P < 0.05), and its pathological damage of organs was relieved and the infiltration of inflammatory cells was alleviated (all P < 0.05). Conclusion Shenfu Injection can regulate the immune system of sepsis mice, inhibit inflammation and alleviate organ damage.
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