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| Study on the therapeutic effect and mechanism of Jiangcan Sicao Decoction on imiquimod-induced psoriasis mice model |
| DUAN Lina1 REN Yu1 JING Huiling2 WANG Xingxing2 WANG Weiying3 |
1.The First Clinical Medical College, Shaanxi University of Chinese Medicine, Shaanxi Province, Xianyang 712046, China;
2.Department of Dermatology, Xi’an Hospital of Traditional Chinese Medicine, Shaanxi Province, Xi’an 710021, China;
3.Department of Nephrology, Xi’an Hospital of Traditional Chinese Medicine, Shaanxi Province, Xi’an 710021, China
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Abstract Objective To investigate the therapeutic effect of Jiangcan Sicao Decoction on imiquimod-induced psoriasis mice model, and to discuss its mechanism. Methods Sixty SPF male BALB/c mice of six to eight weeks were divided into blank group, model group, positive drug control group, Jiangcan Sicao Decoction high-concentration, medium-concentration, and low- concentration groups, with ten mice in each group. Except blank group, the other five groups were treated with 5% Imiquimod Cream 62.5 mg on the back to prepare psorias-like mouse model. Meanwhile, Jiangcan Sicao Decoction high-concentration, medium-concentration, and low-concentration groups were given 20, 10, and 5 mg/kg suspension of Jiangcan Sicao Decoction by intragastric administration, respectively; positive drug control group was given 3.75 mg/kg of distilled water suspension of acitretin by gavage; blank group and model group were given normal saline intragastric administration, once per day for seven consecutive days. The skin area, histopathology, psoriasis area and severity index (PASI) score and serum levels of interleukin (IL)-17A, IL-23, tumor necrosis factor α (TNF-α), growth transforming factor β (TGF-β), and tumor necrosis factor-like weak apoptosis inducer (TWEAK) were compared in each group. Results PASI score in model group was higher than that in blank group (P＜0.05); PASI scores in Jiangcan Sicao Decoction high-concentration, medium-concentration, and low-concentration groups were lower than those in model group (P＜0.05); PASI score of Jiangcan Sicao Decoction medium-concentration group was lower than that of Jiangcan Sicao Decoction low-concentration group, and PASI score of Jiangcan Sicao Decoction high-concentration was higher than that of Jiangcan Sicao Decoction medium-concentration group (P＜0.05). On the 7th day of modeling administration, blank group showed no abnormal symptoms, and the other groups showed different degrees of erythema, scale, and infiltration; compared with model group, the skin lesions in Jiangcan Sicao Decoction group were relatively relieved, the degree of skin lesions in Jiangcan Sicao Decoction group was the lowest. In blank group, the skin layers were clear, the tissue structure was not abnormal, and the epidermal thickness was normal; the skin lesions of model group showed psoriasis like changes, and the degree of keratosis of epidermal cells was reduced in the skin lesions of the mice in the Jiangcan Sicao Decoction group. Serum levels of IL-17A, IL-23, TNF-α, TGF-β, and TWEAK in model group were higher than those in blank group (P＜0.05); the serum levels of IL-17A, IL-23, TNF-α, TGF-β, and TWEAK in Jiangcan Sicao Decoction high-concentration, medium-concentration, and low-concentration groups were lower than those in model group (P＜0.05); the serum levels of IL-17A, IL-23, TNF-α, TGF-β, and TWEAK of mice in Jiangcan Sicao Decoction medium-concentration group were lower than those in Jiangcan Sicao Decoction low-concentration, the serum levels of IL-17A, IL-23, TNF-α, TGF-β, and TWEAK in Jiangcan Sicao Decoction high-concentration group were higher than those in Sicao Decoction medium-concentration group (P＜0.05). Conclusion Jiangcan Sicao Decoction can improve the skin lesions of psoriasis mice model, inhibit the secretion of IL-17A, IL-23, TNF-α, and TGF-β, down-regulate the expression of TWEAK. It may play a therapeutic role by down-regulating TWEAK/Fn14 signal, but the specific mechanism needs further study.
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