Analysis of antibodies IgG and IgM against TORCH-associated pathogens in serum samples
YANG Yuelin1,2 LUO Chun2 HU Dachun3 TANGShurong3 LIU Hongqi1
1.Infection and Immunity Laboratory, Institute of Medical Biology, Chinese Academy of Sciences & Peking Union Medial School, Yunnan Province, Kunming 650118, China;
2.Clinic Laboratory, the First People′s Hospital of Kunming, Yunnan Province, Kunming 650011, China;
3.Department of Obstetrics, the First People′s Hospital of Kunming, Yunnan Province, Kunming 650011, China
Abstract：Objective To provide theoretical guidelines for reasonable application of TORCH assay in aristogenesis and fine rearing through analysis of serum antibody of IgG and IgM against TORCH-associated pathogens (TOX, RV, CMV, HSV1/2) from the pre-pregnant, pregnant patients at early stage and new-born patients. Methods From January of 2014 to December of 2017, 19 533 serum samples were collected from pre-pregnant (pre-pregnant group, 1929 samples), pregnant patients at early stage (pregnant group, 15 555 samples) and new-born patients (new-born group, 2049 samples) in the First People's Hospital of Kunming. ELISA was performed to detect the levels of antibodies IgG and IgM against TORCH-associated pathogens and further determine positive rates of specific antibodies of IgG and IgM. Results ELISA of TORCH antibodies in 19 533 serum samples showed that the positive rate of antibody IgG against any of TORCH-pathogens reached 99.41%. The positive rate of CMV IgG (97.27%) was the highest among four kinds of TORCH-associated pathogens and TOX (3.7%) showed the lowest rate. The positive rate of IgM antibody was 12.8%. The positive rate of HSV1 (6.44%) was the highest and CMV showed the lowest rate (0.39%). Further analysis of the positive rate of IgG by groups showed the more than 99% of IgG positive rate of TORCH-associated pathogens in all groups of prepregnant group, pregnant group at early stage and new born group. The IgM positive rates of TORCH-associated pathogens in pre-pregnant group and pregnant group at early stage, 18.87% and 13.65%, were higher than that in new-born group (0.63%). There were more than 60% of individual serum sample showed the IgG antibodies against 3 TORCH-associated pathogens. However, most of the IgM antibody in individual serum sample was against one of TORCH-associated pathogens. Conclusion The pre-pregnant, pregnant women at early stage and new-born patients are screened for antibodies of IgG and IgM in serum against TORCH-associated pathogens, which is of instructive significance for controlling infections of TORCH pathogens and aristogenesis and fine rearing.
杨悦林1,2 罗纯3 胡大春2 唐树荣2 刘红旗1. 血清中针对TORCH相关病原的IgG和IgM抗体分析[J]. 中国医药导报, 2018, 15(11): 82-85,103.
YANG Yuelin1,2 LUO Chun2 HU Dachun3 TANGShurong3 LIU Hongqi1. Analysis of antibodies IgG and IgM against TORCH-associated pathogens in serum samples. 中国医药导报, 2018, 15(11): 82-85,103.
[1] Nahmias AJ，Josey WE，Naib ZM，et al. Perinatal risk associated with maternal genital herpes simplex virus infection [J]. Am J Obstet Gynecol，1971，110（6）：825-837.
[2] Neu N，Duchon J，Zachariah P. TORCH infections [J]. Clin Perinatol，2015，42（1）：77-103.
[3] 章锦曼，朱宝生.产前诊断技术与规范[J].中国使用妇科与产科杂志，2015，33（90）：803-806.
[4] 倪安平.四种病毒和弓形虫衣原体支原体感染实验室检测的重要性[J].中华检验医学杂志，2003，26（3），133-135.
[5] 孙辉，熊敏，王淑媛.湘潭地区孕妇TORCH检测分析[J].标记免疫分析与临床，2013，20（5）：300-302.
[6] Fatima T，Siddiqui H，Ghildiyal S，et al. Cytomegalovirus infection in pregnant women and its association with bad obstetric outcomes in Northern India [J]. Microb Pathog，2017，113：282-285.
[7] 龚波，张昕民，李俊，等.妇女风疹病毒、弓形虫、巨细胞病毒感染调查分析[J].中国实验诊断学，2011，15（3）：456-472
[8] 黄佩芬，王耀强.番禺区孕前常规健康筛查TORCH的结果及意义[J].检验医学与临床，2013，10（14）：1877-1878.
[9] 王彩凤，张藴.宫内感染与胎儿发育异常[J].中国实用妇科与产科杂志，2002，18（9）：526-529.
[10] Stagno S，Reynolds DW，Amos CS，et al. Auditory and visual defects resulting from symptomatic and subclinical congenital cytomegaloviral and toxoplasma infections [J]. Pediatrics，1977，59（5）：669-678.
[11] Boppana SB，Rivera LB，Fowler KB，et al. Intrauterine transmission of cytomegalovirus to infants of women with preconceptional immunity [J]. N Engl J Med，2001，344（18）：1366-1371.
[12] de Jong EP，Vossen AC，Walther FJ，et al. How to use neonatal TORCH testing？ [J].The New England Journal of Medicine，2013，344（18）：1366-1371.
[13] Nahmias AJ，Norrild B. Herpes simplex viruses 1 and 2-basic and clinical aspects [J]. Dis Mon，1979，25（10）：1-49.
[14] Wolfert SI，de Jong EP，Vossen AC，et al. Diagnostic and therapeutic management for suspected neonatal herpes simplex virus infection [J]. J Clin Virol，2001，51（1）：8-11.
[15] 钱森林，李艳.湖北某地区2356例孕妇TORCH抗体检测分析[J].检验医学与临床，2016，13（4）：522-523.
[16] 倪安平，郝英英，朱晓春，等.孕产妇四种病原体感染血清学筛查的研究[J].中华检验医学杂志，26（3）：142-144.
[17] Pinon JM，Dumon H，Chemla C，et al. Strategy for diagnosis of congenital toxoplasmosis：evaluation of methods comparing mothers and newborns and standard methods for postnatal detection of immunoglobulin G，M，and A ant?鄄ibodies [J]. J Clin Microbiol，2001，39（6）：2267-2271.
[18] 陈艳.1876份TORCGH检测结果分析[J].中国实验诊断学，2014，18（12）：2052-2054.
[19] 徐敏，毛伟，何涛，等.重庆地区健康献血人群弓形虫抗体检测结果分析[J].中国输血杂志，2017，30（1）：58-60.
[20] 崔京涛，马良坤，倪安平.2008至2015年育龄妇女及新生儿TORCH血清学筛查及临床意义分析[J].中华检验医学杂志，2016，39（4）：281-285.
2018, Vol. 15 
京公网安备 11010502046598号