Effects of flavanomarein on oxidative stress and expression of VEGF and ICAM-1 protein in HUVEC cells induced by high glucose
WANG Lili1 LI Linlin1 ZHANG Nannan1 WANG Ye1 LUO Xin1 TAO Yicun1 MAO Xinmin2
1.Department of Pharmacology, College of Pharmacy, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China;
2.College of Traditional Chinese Medicine, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China
Abstract：Objective To observe the effects of flavanomarein glycosides of Coreopsis tinctoria Nutt. on oxidative stress and expression of vascular endothelial growth factor (VEGF) and intercellular cell adhesion molecule-1 (ICAM-1) protein in human umbilical vein endothelial cells (HUVEC) induced by high glucose. Methods The cultured HUVEC cells were divided into normal control group (NG group), high glucose model group (HG group) and different concentrations of flavanomarein group (50, 100, 200, 400 μmol/L). The activity of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in supernatant were detected by enzyme-linked immunosorbent assay (ELASA). The expression levels of VEGF and ICAM-1 protein in cells were detected by Western blot method. Results Compared with NG group, the content of MDA in HG group was significantly increased, the levels of SOD, CAT and GSH-Px in HG group were significantly decreased (P ＜ 0.05 or P < 0.01). Compared with HG group, after flavanomarein intervention, the content of MDA in HG group was significantly decreased, CAT, GSH-Px activity increased significantly (P ＜ 0.05). The results of Western blot showed that compared with NG group, the expression of VEGF and ICAM-1 protein in HG group increased (P ＜ 0.05 or P < 0.01). Compared with HG group, after flavanomarein intervention, the expression of VEGF and ICAM-1 protein increased with the increase of concentration followed by reduction (P ＜ 0.05 or P < 0.01). Conclusion Flavanomarein has a protective effect on HUVEC induced by high glucose may be through improving the level of oxidative stress and decreasing the levels of VEGF and ICAM-1.
王丽丽1 李琳琳1 张楠楠1 王烨1 骆新1 陶义存1 毛新民2. 黄诺玛苷对高糖诱导HUVEC细胞氧化应激及VEGF和ICAM-1蛋白表达的影响[J]. 中国医药导报, 2018, 15(10): 8-12.
WANG Lili1 LI Linlin1 ZHANG Nannan1 WANG Ye1 LUO Xin1 TAO Yicun1 MAO Xinmin2. Effects of flavanomarein on oxidative stress and expression of VEGF and ICAM-1 protein in HUVEC cells induced by high glucose. 中国医药导报, 2018, 15(10): 8-12.
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2018, Vol. 15 
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