优化心肌能量代谢对SMVT大鼠肠道组织中HIF-1与VEGF表达的影响

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摘要目的探讨优化心肌能量代谢对肠系膜上静脉血栓形成大鼠肠道组织中低氧诱导因子-1（HIF-1）与血管内皮生长因子（VEGF）表达的影响。方法将45只健康成年SD大鼠按随机数字表法分为对照组、模型组及治疗组，每组各15只。模型组动物进行造模试验，经皮下注射生理盐水0.2 mL/100 g后常规饲养；治疗组动物经造模后，经尾静脉注射曲美他嗪（10 mg/kg）；对照组则仅打开腹腔，不阻断血运，后经皮下注射生理盐水0.2 mL/100 g。每组均间隔12 h重复给予药物干预，直至术后72 h。观察并比较三组大鼠一般状态、腹腔情况、病理情况及HIF-1、VEGF、丙二醛（MDA）、肌酸激酶（CK）和三磷酸腺苷（ATP）水平。结果对照组一般状况、腹腔情况、肠道组织病理学观察均显示正常，模型组肠道坏死，部分区域伴穿孔，治疗组意识、活动、肠壁血运基本恢复正常，无瘀血水肿及坏死脱落。与对照组比较，模型组HIF-1、VEGF、MDA、CK升高明显，ATP降低明显；与模型组比较，治疗组HIF-1、VEGF、MDA、CK降低明显，ATP升高明显，差异均有统计学意义（P < 0.05）。结论优化能量代谢剂曲美他嗪可清除自由基对心肌细胞的损害，明显降低VEGF、HIF-1水平，改善动物肠道上静脉血栓状况，效果好。

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	虞向阳谢宗源陈建立张国志曹文斌戴亮何艳舫▲

关键词 ：优化心肌能量代谢, 肠系膜上静脉血栓形成, 低氧诱导因子-1, 血管内皮生长因子, 三磷酸腺苷

Abstract：Objective To study the effect of optimized myocardial energy metabolism on the expression of hypoxic induction factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) in rats with superior mesenteric venous thrombosis. Methods Forty-five healthy adult SD rats were divided into control group, model group and treatment group by random number table. The model group animals were tested for the model, which were fed after subcutaneous injection of 0.2 mL/100 g of physiological saline solution. After the animals were formed, the tail vein was injected with Trimetazidine (10 mg/kg) in the treatment group. The control group was only opened the abdominal cavity, but was not block the blood transport, then injected the physiological saline with 0.2 mL/100 g. Each group was given a 12 h interval for drug intervention until 72 h after surgery. General state, abdominal cavity condition and pathological condition were observed, HIF-1, VEGF and malondialdehyde (MDA), creatine kinase (CK), and adenosine triphosphate (ATP) levels were determined. Results The general situation, peritoneal condition and intestinal histopathological observation of control group were normal, while intestinal of the model group was necrosis, the part region was perforation, consciousness and activity of treatment group, blood transport of intestinal was basically normal, and there was no bleeding edema and no necrosis. Compared with the control group, the levels of HIF-1, VEGF, MDA and CK in the model group were significantly higher, ATP level was decreased, with statistically significant difference (P < 0.05). Compared with the model group, the levels of HIF-1, VEGF, MDA and CK in the treatment group were decreased significantly, ATP level was higher, with statistically significant difference (P < 0.05). Conclusion Optimize energy metabolizer Trimetazidine can remove free radical damage to myocardial cells, and can significantly reduce VEGF and hif-1 levels, improve the condition of venous thrombosis in animal intestines with good effect.

Key words： Optimized myocardial energy metabolism Superior mesenteric venous thrombosis Hypoxia-Inducible Factor-1 Vascular endothelial growth factor Adenosine triphosphate

基金资助: 河北省医学研究课题计划（20150519）。

通讯作者: ▲通讯作者

引用本文:

虞向阳谢宗源陈建立张国志曹文斌戴亮何艳舫▲. 优化心肌能量代谢对SMVT大鼠肠道组织中HIF-1与VEGF表达的影响[J]. 中国医药导报, 2018, 15(9): 13-17.
YU Xiangyang XIE Zongyuan CHEN Jianli ZHANG Guozhi CAO Wenbin DAI Liang HE Yanfang▲. Effect of optimized myocardial energy metabolismon on the expression of hypoxic induction factor-1 and vascular endothelial growth factor in rats with superior mesenteric venous thrombosis. 中国医药导报, 2018, 15(9): 13-17.

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