Effects of Yunzhi Wuweizi Extract on liver injury induced by Sulfasalazine in mice
YU Haitao1 WANG Qunying1 MIAO Qing2 DONG Wenzhu1
1.Department of Gastroenterology, No.971 Hospital of Chinese People’s Liberation Army Navy, Shandong Province, Qingdao 266071, China;
2. Department of Pharmacy, No.971 Hospital of Chinese People’s Liberation Army Navy, Shandong Province, Qingdao 266071, China
Abstract：Objective To study the effects of Yunzhi Wuweizi Extract on liver injury induced by Sulfasalazine in mice. Methods Sixty 6-week-old male ICR mice weighing 18-22 g were randomly divided into six groups by numbering method. Control group, model group, Ursodeoxycholic acid group and Yunzhi Wuweizi Extract low, medium, and high dosage groups, with 10 mice in each group. Model group, Ursodeoxycholic acid group and Yunzhi Wuweizi Extract low, medium, and high dose groups were intragastric with Sulfasalazine solution (1000 mg/kg, suspended in 0.5% sodium carboxymethyl cellulose [CMC-Na]) every day to prepare drug liver injury model. Ursodeoxycholic acid group and Yunzhi Wuweizi Extract low, medium, and highdose groups were given ursodeoxycholic acid (10 mg/kg) and Yunzhi Wuweizi Extract solution (50, 100, 300 mg/kg) by intragastric administration 2 h after Sulfadiazine administration, respectively. Model group and control group were given isopyknic 0.5% CMC-Na solution. The intervention lasted for 14 days. On the 15th day, after blood was collected from eyeballs, the serum of each group was separated and determined for glutamic-pyruvic transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), and alkaline phosphatase (ALP). The liver tissues of each group were stained with hematoxylin-eosin (HE) to observe the pathological changes of the liver. Results The levels of GPT, GOT, and ALP in model group were higher than those in control group, and the differences were statistically significant (P ＜ 0.05). The levels of GPT, GOT, and ALP of Yunzhi Wuweizi Extract in low, medium, and high dose groups were lower than those in model group, and those in Yunzhi Wuweizi Extract medium and high dose groups were lower than those in Yunzhi Wuweizi Extract low dose group, with statistical significance (P ＜ 0.05 or P ＜ 0.01). HE staining results showed that hepatic cell swelling, cell membrane detachment and rupture in model group, and liver injury was significantly improved in Ursodeoxycholic acid group and Yunzhi Wuweizi Extract low, medium, and high dose groups. Conclusion Yunzhi Wuweizi Extract has protective effect on liver injury induced by sulfasalazine in mice.
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