Discussion on the mechanism of action of danshen root-fortune’s drynaria rhizome couplet medicines in the treatment of femoral head necrosis based on network pharmacology
1.Graduate School, Hu’nan University of Chinese Medicine, Hu’nan Province, Changsha 410006, China;
2.Department of Orthopedics and Traumatology, Hu’nan Academy of Traditional Chinese Medicine Affiliated Hospital, Hu’nan Province, Changsha 410006, China
Abstract：Objective To explore the mechanism of action of danshen root-fortune’s drynaria rhizome couplet medicines in the treatment of femoral head necrosis based on network pharmacology. Methods Through collecting the active ingredients of danshen root-fortune’s drynaria rhizome from the traditional Chinese medicine systems pharmacology database and analysis platform and screening according to ADME, the potential drug targets were obtained by using UNIPORT database. GeneCards and OMIM databases were used to obtain potential targets of femoral head necrosis, and the intersection of potential targets of drugs and diseases was obtained by R software and Venn diagram was drawn. Cytoscape 3.7.2 was used to construct a compound-target network. Proteinprotein interaction network diagrams were constructed using STRING database. GO functional annotation and KEGG pathway enrichment analysis were carried out for the intersection target of danshen root-fortune’s drynaria rhizome and femoral head necrosis. Results A total of 70 active components and 158 potential targets of danshen root-fortune’s drynaria rhizome couplet medicines were obtained, and 639 related targets of femoral head necrosis were obtained. The active ingredients include luteolin, kaempferol, tanshinone ⅡA, naringenin, β-sitosterol, etc. Eleven key targets, IL-6, AKT1, TP53, VEGFA, JUN, MAPK8, TNF, MAPK3, PTGS2, CASP3, and MAPK1 were identified after screening. GO function enrichment showed that the molecular functions of the target mainly involve inflammation, cell metabolism, nuclear chromatin, protein, enzyme, phosphatase receptor binding, etc. Enrichment analysis of KEGG pathway revealed 163 channels, which were mainly related to PI3K-Akt, MAPK, IL-17 and cancer-related pathways after further screening. Analysis of genes in KEGG pathway showed that almost every pathway involved core targets such as AKT, VEGFA, TNF and MAPK. Conclusion Danshen root-fortune’s drynaria rhizome couplet medicines may play a role in the treatment of femoral head necrosis through targets such as AKT, VEGFA, TNF, MAPK and pathways such as PI3K-Akt, MAPK, IL-17 and cancer.
陈中1 郑阳1 仇湘中2 张信成2 尹晨东1. 基于网络药理学探究丹参-骨碎补药对治疗股骨头坏死的作用机制[J]. 中国医药导报, 2021, 18(24): 17-21,39.
CHEN Zhong1 ZHENG Yang1 QIU Xiangzhong2 ZHANG Xincheng2 YIN Chendong1. Discussion on the mechanism of action of danshen root-fortune’s drynaria rhizome couplet medicines in the treatment of femoral head necrosis based on network pharmacology. 中国医药导报, 2021, 18(24): 17-21,39.
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