Abstract:Objective To improve the quality standard of Baibo Pills and to provide scientific basis for improving its quality standard. Methods Microscopical identification method was used to identify puncturevine caltrop fruit, divaricate saposhnikovia root, suberect spatholobus stem, safflower and heidou. Thin layer chromatography (TLC) was used to identify chinese angelica, tuber fleeceflower stem and peony root. The AgilentSB-C18 octaneylsilane bonded silica gel column (250 mm×4.6 mm, 5 μm) was used with gradient elution. The column temperature was 40℃, the flow rate was 1.0 ml/min, and the mobile phase was methanol-water (55∶45). The detection wavelength was 246 nm. The content of isopsoralen and psoralen in Baibo Pills was determined. Results The microscopic characteristics of puncturevine caltrop fruit, divaricate saposhnikovia root, suberect spatholobus stem, safflower and heidou were obvious. TLC spots of chinese angelica, tuber fleeceflower stem and peony root were clear, and negative control had no interference. The linear range of psoralen was 0.0204-0.3066 μg (r = 0.9999). The average recovery was 98.08%, relative standard deviation (RSD) = 1.07%; The linear range of isopsoralen was 0.0204-0.3054 μg (r = 0.9998). The average recovery was 97.92%, RSD = 1.99%. Conclusion The method is comprehensive, accurate and reproducible, which can improve the quality control of Baiba Pills.