口腔扁平苔藓病损区Tim-3、Gal-9、IFN-γ的表达及临床意义

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摘要目的探讨T细胞免疫球蛋白及黏蛋白结构域蛋白-3（Tim-3）、半乳糖凝集素-9（Gal-9）、γ干扰素（IFN-γ）在口腔扁平苔藓（OLP）组织中的表达及Tim-3/Gal-9通路在OLP病损形成中的作用及意义。方法选择2016年10月至2019年10月贵州医科大学附属口腔医院（以下简称“我院”）牙周黏膜科诊治的OLP患者41例为OLP组，选取同期于我院诊治的10名正常口腔黏膜人群作为正常组。采用免疫组织化学SABC法检测两组Tim-3、Gal-9、IFN-γ的表达情况及相关性，同时分析OLP患者病理特征与Tim-3、Gal-9、IFN-γ的相关性。结果 OLP组Tim-3、IFN-γ阳性表达率高于正常组，Gal-9阳性表达率低于正常组，差异有统计学意义（P ＜ 0.05）。OLP组织中，Tim-3表达在上皮层、固有层呈正相关（r > 0，P < 0.05）；Tim-3与Gal-9表达在上皮层呈负相关（r < 0，P ＜ 0.05）；Tim-3和IFN-γ表达在固有层呈正相关（r > 0，P ＜ 0.05）。淋巴细胞浸润记分与Tim-3在上皮层与固有层的阳性表达呈正相关（r > 0，P ＜ 0.05），与Gal-9呈负相关（r < 0，P ＜ 0.05）；基底细胞液化浸润记分与Tim-3在上皮层阳性表达、IFN-γ阳性表达呈正相关（r > 0，P ＜ 0.05）。结论 OLP患者病损区中存在Tim-3高表达和Gal-9低表达，可能是由于Tim-3/Gal-9通路异常，使辅助性T细胞1效应细胞增强，促进IFN-γ分泌，参与了OLP病损区病理损伤的过程。

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	王珣1 王冬平2 蔡扬1

关键词 ：口腔扁平苔藓, T细胞免疫球蛋白及黏蛋白结构域蛋白-3, 半乳糖凝集素-9, &gamma, 干扰素, 免疫组织化学

Abstract：Objective To investigate the expression of T cell immunoglobulin domain and mucin domain protein-3 (Tim-3), galectin-9 (Gal-9) and interferon-γ (IFN-γ) in oral lichen planusand (OLP) tissues and the role of Tim-3/ Gal-9 pathway in the formation of OLP and its significance. Methods From October 2016 to October 2019, 41 cases of OLP patients treated in the Department of Periodontal Mucosa, Stomatological Hospital of Guizhou Medical University (“our hospital” for short) were selected as the OLP group, and 10 normal oral mucosal people treated in our hospital during the same period were selected as the normal group. The expression of Tim-3, Gal-9, and IFN-γ in the two groups and their correlation were detected by immunohistochemical SABC method, at the same time, the correlation between the pathological characteristics of OLP patients and Tim-3, Gal-9 and IFN-γ were analyzed. Results The positive expression rates of Tim-3 and IFN-γ in the OLP group were higher than those in the normal group, and the positive expression rates of Gal-9 was lower than that in the normal group, with statistical significance (P < 0.05). In OLP tissue, the expression of Tim-3 was positively correlated with the epithelium and lamina propria (r > 0, P < 0.05); the expression of Tim-3 and Gal-9 was negatively correlated in the epidermis (r < 0, P < 0.05); and the expression of Tim-3 and IFN-γ was positively correlated in lamina propria (r > 0, P < 0.05). The score of lymphocyte infiltration was positively correlated with the positive expression of Tim-3 in the epithelium and lamina propria (r > 0, P < 0.05), and negatively correlated with Gal-9 (r < 0, P < 0.05); the score of liquefaction infiltration of basal cells was positively correlated with the positive expression of Tim-3 and IFN-γ in the epidermis (r > 0, P < 0.05). Conclusion The high expression of Tim-3 and low expression of Gal-9 in the lesions of OLP patients may be due to the abnormal Tim-3/Gal-9 pathway, which enhances T helper cell 1 effector cells and promotes the secretion of IFN-γ, and is involved in the process of pathological injury in the lesion area of OLP.

Key words： Oral lichen planus T cell immunoglobulin domain and mucin domain protein-3 Galectin-9 Interferon-γ Immunohistochemistry

基金资助:国家自然科学基金资助项目（81460104）；
贵州医科大学附属口腔医院科研基金项目[GYKQKY（2020-04号）]。

通讯作者: 蔡扬（1963.3-），女，博士，教授，主任医师；研究方向：口腔黏膜病的免疫发病机制。

作者简介: 王珣（1989.3-），女，硕士；研究方向：口腔黏膜病的免疫发病机制。

引用本文:

王珣1 王冬平2 蔡扬1. 口腔扁平苔藓病损区Tim-3、Gal-9、IFN-γ的表达及临床意义[J]. 中国医药导报, 2021, 18(21): 8-11.
WANG Xun1 WANG Dongping2 CAI Yang1. Expression and clinical significance of Tim-3, Gal-9 and IFN-γ in oral lichen planus lesions. 中国医药导报, 2021, 18(21): 8-11.

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https://www.yiyaodaobao.com.cn/CN/ 或 https://www.yiyaodaobao.com.cn/CN/Y2021/V18/I21/8

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