Network pharmacological study on the mechanism of action of Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma couplet medicines on the intervention of ulcerative colitis
DAI Mingming1 LIAO Dan2 CHENG Weiling1 WANG Chunpeng1 LIN Caizhi3
1.Graduate School, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530000, China;
2.School of Basic Medicine, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530000, China;
3.Department of Spleen, Stomach and Liver Diseases, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530000, China
Abstract：Objective To analyze the mechanism of action of Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma couplet medicines on the intervention of ulcerative colitis (UC) based on network pharmacology. Methods The active components and target genes of Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma were screened by traditional Chinese medicine systems pharmacology database and analysis platform and bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine. UC-related genes were obtained using GeneCards, a human gene database. Cytoscape software was used to build the “drug-active component-target-disease” network. Proteinprotein interaction network diagrams of Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma couplet medicines on disease targets were constructed by String database. GO functional enrichment and KEGG pathway enrichment analysis of key target genes were performed using R language software. Results A total of 16 active ingredients were screened out. A total of 4209 UC-related genes were retrieved. A total of 31 common targets of couplet medicines and disease-related were screened out. The main core genes included E1A binding protein P300, tumor necrosis factor-α, mitogen-activated protein kinase 1 (MAPK1), etc. The main signaling pathways included phosphoinositol-3 kinase/protein kinase B, nuclear factor-κB, human T-cell leukemia virus type 1, MAPK, hypoxia-inducible factor-1, etc. Conclusion By building a “drug-active component-target-disease” network, from multiple components, multiple targets, multiple pathways to interpret mechanism of action of Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma couplet medicines to intervene in the UC, provide the basis for subsequent research and reference.
戴明明1 廖丹2 程伟玲1 王春鹏1 林才志3. 白术-苍术药对干预溃疡性结肠炎作用机制的网络药理学研究[J]. 中国医药导报, 2021, 18(20): 4-9.
DAI Mingming1 LIAO Dan2 CHENG Weiling1 WANG Chunpeng1 LIN Caizhi3. Network pharmacological study on the mechanism of action of Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma couplet medicines on the intervention of ulcerative colitis. 中国医药导报, 2021, 18(20): 4-9.
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