Study on the anesthetic effect of new type topical anesthetic on oral mucosa surface
ZENG Dong1 NIU Jiao2 LI Changyi3 CHANG Jin4 NIU Guangliang1▲
1.Beijing Hospital of Integrated Traditional Chinese and Western Medicine，Beijing 100039, China;
2.Second Hospital of Shanxi Medical University, Shanxi Province, Taiyuan 030001, China;
3.Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China;
4.Institute of Nanomedicine, School of Life Sciences, Tianjin University, Tianjin 300072, China
Abstract：Objective To investigate the anesthetic effect of new type topical anesthetic which is Lidocaine hydrochloride lysine-linoleic acid modified dextran polymeric lipid vesicles on oral mucosa surface. Methods Eighteen New Zealand rabbits (male or female, body weight [2.0±0.2] kg) aged eight months were divided into negative control group (0.5 ml of Lidocaine hydrochloride injection was applied on the labial gingival of the lower anterior teeth)，positive control group (0.5 ml of Lidocaine hydrochloride conventional liposomes was applied on the labial gingival of the lower anterior teeth) and experimental group (0.5 ml of Lidocaine hydrochloride lysine-linoleic acid modified dextran polymeric lipid vesicles was applied on the labial gingival of the lower anterior teeth) by stratified random grouping according to body weight, with six rats in each group. Acupuncture experiment and mental nerve action potential experiment were performed in the three groups, and the experimental results were compared. Results The results of acupuncture experiment showed that the onset time of anesthesia in the experimental group was shorter than that in the positive control group, and the difference was statistically significant (all P ＜ 0.05). No significant anesthetic effect was found in negative control group. Overall analysis of the inhibition rate of mental nerve action potential amplitude (APA) among the three groups showed that there were statistically significant differences among groups, time point comparison and interaction (P ＜ 0.05). Comparison within groups: the inhibition rate of APA at 50 min in negative control group and positive control group was higher than that at other time points, with statistical significance (all P ＜ 0.05); the inhibition rate of APA in experimental group at 40 min was higher than that at other time points, with statistical significance (all P ＜ 0.05). Comparison between groups: the APA inhibition rate of the experimental group was higher than that of the positive control group at each time point, and the APA inhibition rate of the positive control group was higher than that of the negative control group within 50 min, with statistical significance (all P ＜ 0.05). Conclusion Lidocaine hydrochloride lysine-linoleic acid modified dextran polymeric lipid vesicles has a quick effect and good anesthetic effect, which has a good clinical application prospect.
曾东1 牛娇2 李长义3 常津4 牛光良1▲. 新型表面麻醉剂的口腔黏膜表面麻醉效果研究[J]. 中国医药导报, 2021, 18(19): 36-39,59.
ZENG Dong1 NIU Jiao2 LI Changyi3 CHANG Jin4 NIU Guangliang1▲. Study on the anesthetic effect of new type topical anesthetic on oral mucosa surface. 中国医药导报, 2021, 18(19): 36-39,59.
[1] Avou O，Rehan，SM，Sawy EI，et al. Lidocaie/β-cyclodextrin inclusion complex as drug delivery system [J]. Eur Polym J，2018（8）：304-310.
[2] Wang Y，Su W，Li Q，et al. Preparation and evaluation of lidocaine hydrochloride-loaded TAT-conjugated polymeric liposomes for transdermal delivery [J]. Int J Pharm，2013，441（1/2）：748-756.
[3] 刘冰滢，刘伟.表面麻醉剂在瘢痕局部非手术治疗中的应用进展[J].组织工程与重建外科杂志，2019，15（1）：58-60.
[4] Lacerda-Santos R，Bravin TC，Carvalho FG，et al. Efficacy of Topical Anesthetics in Pain Perception During Mini-implant Insertion：Systematic Review of Controlled Clinical Trials [J]. Anesth Prog，2019，66（3）：119-132.
[5] Ribeiro LNM，Franz-Montan M，Alc?覾ntara ACS，et al. Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry [J]. Sci Rep，2020，10（1）：11341.
[6] 徐浩，申旭霁，栾晶，等.中药口腔黏膜表面麻醉及临床应用[J].实用口腔医学杂志，2020，36（2）：385-388.
[7] Wang S，Zeng D，Niu J，et al. Development of an efficient transdermal drug delivery system with TAT-conjugated cationic polymeric lipid vesicles [J]. J Mater Chem B，2014， 2（7）：877-884.
[8] 牛娇，曾东，李芹，等.盐酸利多卡因葡聚糖基纳米类脂质体的透皮研究[J].天津医药，2015，43（4）：353-355.
[9] Bahramizadeh M，Bahramizadeh M，Kiafar B，et al. Development，characterization and evaluation of topical meth-otrexate-entrapped deformable liposome on imiquimod-induced psoriasis in a mouse model [J]. Int J Pharm，2019，569：118623.
[10] Wang SB，Liu XH，Li B，et al. Bacteria-Assisted Selective Photothermal Therapy for Precise Tumor Inhibition [J]. Adv Funct Mater，2019，29（35）：11-14.
[11] Ding R，Li Z，Wang J，et al. Design and Synthesis of Galactose-Biotin Lipid Materials for Liposomes to Promote the Hepatoma Cell-Targeting Effect [J]. J Pharm. Sci，2019，108（9）：3074-3081.
[12] Powell MF，Jarzombek KW，Venhuizen KJ，et al. Comparing Bupivacaine，Lidocaine，and a Combination of Bupivacaine and Lidocaine for Labor Epidural Activation：A Prospective，Randomized，Double-Blind Study [J]. Asian J Anesthesiol，2019，57（2）：55-60.
[13] de Almeida ACP，Pinto LMA，Alves GP，et al. Liposomal-based lidocaine formulation for the improvement of infiltrative buccal anaesthesia [J]. J Liposome Res，2019， 29（1）：66-72.
[14] Monika B，Szilvia B，Maria BS，et al. DSC for evaluating the encapsulation efficiency of lidocaine-loaded liposomes compared to the ultracentrifugation method [J]. Therm Anal Calorim，2017，130（3）：1619-1625.
[15] Wang J，Zhang L，Chi H，et al. An alternative choice of lidocaine-loaded liposomes：lidocaine-loaded lipid-polymer hybrid nanoparticles for local anesthetic therapy [J]. Drug Deliv，2016，23（4）：1254-1260.
[16] Cevc G. Transfersomes，liposomes and other lipid suspensions on the skin：permeation enhancement，vesicle penetration，and transdermal drug delivery [J]. Crit Rev Ther Drug Carrier Syst，1996，13（3/4）：257-388.
[17] Huang W，Vidimos A. Topical anesthetics in dermatology [J]. J Am Acad Dermatol，2004，43（2 Pt 1），286-298.
[18] Pellicer-Chover H，Cervera-Ballester J，Sanchis-Bielsa JM，et al. Comparative split-mouth study of the anesthetic efficacy of 4% articaine versus 0.5% bupivacaine in impacted mandibular third molar extraction [J]. J Clin Exp Dent，2013，5（2）：e66-e71.
[19] Cohen H，Reader A，Drum M，et al. Anesthetic efficacy of a combination of 0.9% M mannitol plus 68.8 mg of lidocaine with 50 μg epinephrine in inferior alveolar nerve blocks： a prospective randomized，single blind study [J]. Anesth Prog，2013，60（4）：145-152.
[20] Al-Haideri YA. Comparison of local anesthetic efficacy of tramadol hydrochloride（with adrenaline）versus plain tramadol hydrochloride in the extraction of upper molar teeth [J]. J Oral Maxillofac Surg，2013，71（12）：2035-2038.
[21] Su N，Li C，Wang H，et al. Efficacy and safety of articaine versus lignocaine in dental treatments：A meta-analysis [J]. Aust Endod J，2016，42（1）：4-15.
[22] 徐淑云.药理学实验方法（第三版）[M].北京：人民卫生出版社，2002.
[23] Takeda M，Oshima K，Takahashi M，et a. Systemic administration of lidocaine suppresses the excitability of rat cervical dorsal horn neurons and tooth-pulp-evoked jaw-opening reflex [J]. Eur J Pain，2009，13（9）：929-934.
[24] 张长梅，陈重，唐雪枫，等.表面修饰糖类化合物的纳米粒子主动靶向肿瘤的研究进展[J].中国医药导报，2019， 16（34）：61-64.
[25] 赵爽，张少芳，穆晓宇.纳米药物的研究进展[J].天津药学，2020，32（2）：57-61.
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