Experimental study of TLR4/NF-κB signal pathway in the mechanism of incisional pain
LIU Dehua1 WANG Jian2 GAO Zhiqiang1 ZOU Liansheng1 XU Ying3
1.Deaprtment of Neurosurgery, First Affiliated Hospital of Gannan Medical University, Jiangxi Province, Ganzhou 341000, China;
2.Department of Pathology, Ganzhou People′s Hospital, Jiangxi Province, Ganzhou 341000, China;
3.Scientific Research Center, Gannan Medical University, Jiangxi Province, Ganzhou 341000, China
Abstract：Objective To investigate the mechanism of Toll like receptor 4 (TLR4)/ nuclear factor κB (NF-κB) signal pathway in rats with incisional pain. Methods One hundred healthy adult male Sprague-Dawley (SD) SPF rats of 20 weeks old were selected to establish incision pain models. Before operation and after operation for 0.5 h, 1 h, 2 h, 8 h, 12 h, 1 d, 2 d, 3 d, 5 d, 7 d, the mechanism withdraw threshold (MWT) in non-operative side and operative side of rats were detected by electronic von Frey pressure pain threshold detector. Before operation and after operation for 2 h, 8 h, 1 d, 2 d, 3 d, 5 d and 7 d, the expression of TLR4, NF-κB, interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) in the spinal cord were detected by real-time fluorescence quantitative PCR (real-time PCR). Results After operation, the MWT in the operative side of rats was lower than that before operation, which was the lowest after operation for 2 h, then increased gradually, and they were significantly lower than those of non-operative side (P ＜ 0.05). After operation, the expression levels of TLR4, NF-κB, IL-1β and TNF-α in spinal cord of rats were increased compared with those before operation, which of postoperative 2 h, 8 h, 1 d, 2 d and 3 d had statistically significant differences compared with those before operation (P ＜ 0.05), which of postoperative 5 d and 7 d had no statistically significant differences compared with those before operation (P ＞ 0.05). The expression levels of TLR4 and NF-κB in the spinal cord reached the highest after operation for 1 d, and the expression levels of IL-1β and TNF-α reached the highest after operation for 8 h. Pearson correlation analysis showed that there was a negative correlation of MWT in operative side with the expression of TLR4 and NF-κB (r = -0.473, P ＜ 0.05, r = -0.398； P ＜ 0.05), the TLR4 levels in spinal cord of rats were positively correlated with the expression levels of IL-1β and TNF-α (r = 0.302, P ＜ 0.05; r = 0.538, P ＜ 0.05), and the NF-κB levels in spinal cord of rats were positively correlated with the expression levels of IL-1β and TNF-α (r = 0.283, P ＜ 0.05; r = 0.467, P ＜ 0.05). Conclusion The signal pathway of TLR4/NF-κB in spinal cord of rats with incisional pain is activated, the expression levels of TLR4 and NF-κB are increased, and the expression levels of downstream inflammatory cytokines IL-1β and TNF-α are up-regulated, which is involved in the regulation process of incisional pain.
刘德华1 王建2 高志强1 邹连生1 徐鹰3 . TLR4/NF-κB信号通路在切口痛作用机制中的实验研究[J]. 中国医药导报, 2018, 15(4): 23-26.
LIU Dehua1 WANG Jian2 GAO Zhiqiang1 ZOU Liansheng1 XU Ying3 . Experimental study of TLR4/NF-κB signal pathway in the mechanism of incisional pain. 中国医药导报, 2018, 15(4): 23-26.
2018, Vol. 15 
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