hBD-3和NF-κB p65在口腔潜在恶性病变和口腔鳞状细胞癌中的表达及意义

摘要
图/表
参考文献(25)
相关文章 (15)

全文: PDF (755 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的检测β-防御素-3（hBD-3）和核因子-κB（NF-κB）p65在正常口腔黏膜、口腔潜在恶性病变（OPMD）及口腔鳞状细胞癌（OSCC）中的表达并探讨相关性。方法收集中南大学湘雅医学院附属海口医院病理科2015年1月—2018年12月60例OPMD患者、40例OSCC患者及15例口腔黏膜正常者的临床资料及组织学标本，采用免疫组织化学法检测各组病理组织中hBD-3与NF-κB p65的表达，分析在不同类型组织标本中的差异，以及两种蛋白之间的相关性。结果 OSCC中hBD-3、NF-κB p65的表达强度高于正常组及OPMD组（P ＜ 0.05），OPMD组高于正常组（P ＜ 0.05）。hBD-3与NF-κB p65的表达呈正相关（r = 0.355，P ＜ 0.05）。hBD-3、NF-κB p65与OPMD上皮异常增生相关（P ＜ 0.05）。结论 hBD-3和NF-κB p65蛋白在口腔黏膜早期癌变过程中表达增高，并有明显协同作用，两者可能参与了口腔鳞癌的发生发展。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	杜永秀杨琴杨晨希徐普

关键词 ： &beta, -防御素-3, 核因子-&kappa, B p65, 口腔潜在恶性病变, 口腔鳞状细胞癌

Abstract：Objective To investigate the expression and correlation of human β-defensin-3 (hBD-3) and nuclear factor kappaB (NF-κB) p65 in normal oral mucosa, oral potentially malignant disorder (OPMD), and oral squamous cell carcinoma (OSCC). Methods Clinical data and histological specimen from the Department of Pathology, Haikou Affiliated Hospital of Center South University Xiangya School of Medicine from January 2015 to December 2018, 60 patients with OPMD, 40 patients with OSCC and 15 cases of normal oral mucosa were collected. Immunohistochemical staining was applied to investigate the expression of hBD-3 and NF-κB p65 in paraffin sections of normal oral mucosa, OPMD, and OSCC tissues. The expression in different types of tissue specimens and their correlation were analyzed. Results The expression intensity of hBD-3 and NF-κB p65 in OSCC was significantly higher than those in normal group and OPMD group (P ＜ 0.05), and OPMD group was significantly higher than that in normal group (P < 0.05). The expression of hBD-3 was positively correlated with NF-κB p65 (r = 0.355，P ＜ 0.05). HbD-3 and NF-κB p65 were associated with OPMD epithelial dysplasia (P ＜ 0.05). Conclusion The expression of hBD-3 and NF-κB p65 protein is increased in the early carcinogenesis of oral mucosa and has obvious synergistic effect, suggesting that they may be involved in the occurrence and development of oral squamous cell carcinoma.

Key words： Human β-defensin-3 Nuclear factor-κB p65 Oral potentially malignant disorder Oral squamous cell carcinoma

基金资助:海南省自然科学基金资助项目（817381）；
海南省卫生计生行业科研项目（18A200071）。

通讯作者: 徐普（1963.4-），男，博士，主任医师；研究方向：口腔种植。

作者简介: 杜永秀（1979-），女，硕士，副主任医师；研究方向：口腔黏膜潜在恶性疾病。

引用本文:

杜永秀杨琴杨晨希徐普. hBD-3和NF-κB p65在口腔潜在恶性病变和口腔鳞状细胞癌中的表达及意义[J]. 中国医药导报, 2021, 18(17): 25-28,35.
DU Yong xiu YANG Qin YANG Chenxi XU Pu. Expression of hBD-3 and NF-κB p65 in oral potentially malignant disorder and oral squamous cell carcinoma . 中国医药导报, 2021, 18(17): 25-28,35.

链接本文:

https://www.yiyaodaobao.com.cn/CN/ 或 https://www.yiyaodaobao.com.cn/CN/Y2021/V18/I17/25

[1] Thomson PJ. Perspectives on oral squamous cell carcinoma prevention，proliferation，position，progression and prediction [J]. J Oral Pathol Med，2018，47（9）：803-807.
[2] Global Burden of Disease Cancer Collaboration，Fitzmaurice C，Abate D，et al. Global，Regional，and National Cancer Incidence，Mortality，Years of Life Lost，Years Lived With Disability，and Disability-Adjusted LifeYears for 29 Cancer Groups，1990 to 2017：A Systematic Analysis for the Global Burden of Disease Study [J]. JAMA Oncol，2019，5（12）：1749-1768.
[3] Dumache R. Early Diagnosis of Oral Squamous Cell Carcinoma by Salivary microRNAs [J]. Clin Lab，2017，63（11）：1771-1776.
[4] Oliveira ML，Wagner VP，Sant’ana Filho M，et al. A 10-year analysis of the oral squamous cell carcinoma profile in patients from public health centers in Uruguay [J]. Braz Oral Res，2015，29（1）：1-8.
[5] Nadeau C，Kerr AR. Evaluation and Management of Oral Potentially Malignant Disorders [J]. Dent Clin North Am，2018，62（1）：1-27.
[6] Chen L，Lv Z，Gao Z，et al. Human β-defensin-3 reduces excessive autophagy in intestinal epithelial cells and in experimental necrotizing enterocolitis [J]. Sci Rep，2019，9（1）：19890.
[7] Gomez Hernandez MP，Bates AM，Starman EE，et al. HBD3 Induces PD-L1 Expression on Head and Neck Squamous Cell Carcinoma Cell Lines [J]. Antibiotics （Basel），2019， 8（4）：161.
[8] Banke IJ，Stade N，Prodinger PM，et al. Antimicrobial peptides in human synovial membrane as （low-grade） periprosthetic joint infection biomarkers [J]. Eur J Med Res，2020， 25（1）：33.
[9] Monkkonen T，Debnath J. Inflammatory signaling cascades and autophagy in cancer [J]. Autophagy，2018：14（2）：190-198.
[10] Taniguchi K，Karin M. NF-κB，inflammation，immunity and cancer：coming of age [J]. Nat Rev Immunol，2018， 18（5）：309-324.
[11] Xu D，Zhang B，Liao C，et al. Human beta-defensin 3 contributes to the carcinogenesis of cervical cancer via activation of NF-kappaB signaling [J]. Oncotarget，2016， 7（46）：75902-75913.
[12] 朱蓉，翦新春，刘德裕，等.PTPRZ1在口腔黏膜下纤维性变癌变中的表达及临床意义[J]. 上海口腔医学，2017，26（2）：198-203.
[13] Speight PM，Khurram SA，Kujan O. Oral potentially malignant disorders：risk of progression to malignancy [J]. Oral Surg Oral Med Oral Pathol Oral Radiol，2018，125（6）：612-627.
[14] Judge CJ，Reyesaviles E，Conry SJ，et al. HBD-3 induces NK cell activation，IFN-γ secretion and mDC dependent cytolytic function [J]. Cellular Immunology，2015，297（2）：61-68.
[15] Ghosh SK，Mccormick TS，Weinberg A. Human Beta Defensins and Cancer：Contradictions and Common Ground [J]. Front Oncol，2019，9：341.
[16] Winter J，Pantelis A，Reich R，et al. Human beta-defensin-1，-2，and -3 exhibit opposite effects on oral squamous cell carcinoma cell proliferation [J]. Cancer Invest，2011，29（3）：196-201.
[17] Shuyi Y，Feng W，Jing T，et al. Human beta-defensin-3（hBD-3） upregulated by LPS via epidermal growth factor receptor （EGFR） signaling pathways to enhance lymphatic invasion of oral squamous cell carcinoma [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod，2011， 112（5）：616-625.
[18] Jin G，Kawsar HI，Hirsch SA，et al. An antimicrobial peptide regulates tumor-associated macrophage trafficking via the chemokine receptor CCR2，a model for tumorigenesis [J]. PLoS One，2010，5（6）：e10993.
[19] Kawsar HI，Weinberg A，Hirsch SA，et al. Over expression of human beta-defensin-3 in oral dysplasia：potential role in macrophage trafficking [J]. Oral Oncol，2009，45（8）：696-702.
[20] Kamperos G，Nikitakis N，Sfakianou A，et al. Expression of NF-kappaB and IL-6 in oral precancerous and cancerous lesions：An immunohistochemical study [J]. Med Oral Patol Oral Cir Bucal，2016，21（1）：e6-e13.
[21] 周骢，伍宝琴，张齐梅，等.NF-κB p65、EGF与EGFR在口腔扁平苔藓和口腔鳞癌中的表达及临床意义[J].重庆医学，2017（2）：187-190.
[22] Qian Y，Jiang W，Wang X，et al. Expression of nucleotide-binding oligomerization domain 1，nuclear factor-kappa B and human beta-defensins in candidal albicans leukoplakia [J]. Zhonghua Kou Qiang Yi Xue Za Zhi，2014，49（8）：464-469.
[23] Phan TK，Lay FT，Hulett MD. Importance of phosphoinositide binding by human beta-defensin 3 for Akt-dependent cytokine induction [J]. Immunol Cell Biol，2018，96（1）：54-67.
[24] Jia J，Peng H，Chen S. Evaluation of the role of human β-defensin 3 in modulation of immunity and inflammatory response after knee replacement [J]. Exp Ther Med. 2017，13（4）：1343-1346.
[25] Mburu YK，Abe K，Ferris LK，et al. Human β-defensin 3 promotes NF-kappaB-mediated CCR7 expression and anti-apoptotic signals in squamous cell carcinoma of the head and neck [J]. Carcinogenesis，2011，32（2）：168-174.