Effects of reinforcing-reducing method by twirling of acupuncture on contents of malondialdehyde and glutathione peroxidase in hippocampus of spontaneously hypertensive rats
Abstract：Objective To explore the effects of reinforcing-reducing method by twirling of acupuncture on contents of malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in hippocampus of spontaneously hypertensive rats. Methods A total of 18 spontaneously hypertensive rats were divided into three groups by random number table method: reducing method group, reinforcing method group, and model group, with six rats in each group. Six WKY rats were served as blank group. All rats were SPF grade, nine-week-old male rats. After one week of acclimatization, reducing method group and reinforcing method group were intervened with corresponding manipulation of reducing and reinforcing on bilateral tàichōng once a day for 14 days. Model group and blank control group received the same grasping and fixation stimulation at the same points of reinducing method group and reinforcing method group. Hippocampus samples were collected on the 15th day, and the morphology of hippocampus was observed by HE staining, MDA content was detected by thiobarbituric acid method and GSH-Px content was detected by colorimetry. Results HE staining results showed that the cells in the blank group had complete structure, regular morphology, normal intercellular space, most nuclei were deeply stained, nucleoli were clear, and no denatured necrosis was observed. Compared with the blank group, the staining in the model group was significantly lighter, and nuclear pyknosis, cell lysis and fragmentation, vacuolar degeneration and apoptotic cells were observed. Compared with the model group, the cell contour was clear, the cell staining was deep, the nucleoli were clear, and the cell structure was basically normal in both the reducing method group and reinforcing method group. MDA content in model group was higher than that in blank group, while GSH-Px content in model group was lower than that in blank group, and the differences were highly statistically significant (P ＜ 0.01). MDA contents in reducing method group and reinforcing method group were lower than those in model group, the contents of GSH-Px in the reducing method group and reinforcing method group were higher than those in the model group, and the differences were statistically significant (P < 0.05 or P ＜ 0.01). Conclusion Reinforcing-reducing method by twirling of acupuncture can regulate the expression level of MDA and GSH-Px in the hippocampus of spontaneously hypertensive rats, inhibit the oxidative reaction, and reduce the damage of oxygen radicals to the hippocampus of spontaneously hypertensive rats, which may be one of the protective mechanisms of reinforcing-reducing method by twirling of acupuncture on hypertensive hippocampal injury.
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