Abstract：Objective To analyze the homology of 29 New Delhi Meta-β-lactamase 1 (NDM-1)-producing Enterobacteriaceae strains, and to understand the prevalence of NDM-1 in Zhejiang Province, and the surrounding environment of blaNDM-1 gene was elucidated by sequence analysis. Methods The drug resistance genes of 29 NDM-1-producing Enterobacteriaceae strains isolated from Zhejiang Hospital were amplified by PCR; the plasmid types were analyzed based on PCR-based plasmid replicon typing (PBRT), and the surrounding sequence of blaNDM-1 gene was amplified by PCR to analyze the similarities and differences of the surrounding sequence. Results Among the 29 blaNDM-1-carrying strains, 86.9% (26/29) were associated with extended spectrum beta-lactamases or other carbapenemase resistance genes; PFGE results showed that six strains of Enterobacter aerogenes, five strains of Enterobacter cloacae, two strains of Escherichia coli and two strains of Klebsiella oxytoca had homology, respectively. Multilocus sequence typing found that three strains of Escherichia coli were ST167 and five strains of Enterobacter cloacae were ST114. The conjugation experiments showed that 27 strains were successfully conjugated; 16 strains were found to be IncX3 type by PBRT plasmid typing, and 16 strains were found to carry blaNDM-1-bleMBL-trpF gene fragment by analyzing the surrounding environment of blaNDM-1. Conclusion NDM-1 has become an important cause of carbapenem antibiotic resistance in Zhejiang Province. IncX3 plasmids are widely spread among different bacterial species, and blaNDM-1-bleMBL-trpF gene fragments are widely disseminated in NDM-1-producing strains.
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