囊型包虫病所致过敏反应对肠黏膜屏障功能的影响

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摘要目的探讨囊型包虫病所致过敏反应对肠黏膜屏障功能的影响。方法 45只6～8周龄雌性BALB/c小鼠，SPF级，体重18～22 g，采用随机数字表法将其分成空白对照组、囊型包虫病模型组、囊型包虫病致敏组，每组各15只；囊型包虫病模型组和囊型包虫病致敏组小鼠通过腹腔注射细粒棘球蚴微囊，每只小鼠接种约50个微囊的剂量，建立包虫病小鼠模型。饲养3个月后，囊型包虫病致敏组注射粗制包虫囊液引发过敏反应，空白对照组和囊型包虫病模型组注射等量生理盐水。检测各组小鼠肠组织中白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）和磷酸化核转录因子kappa B-p65（p-NF-κBp65）的含量及血浆中二胺氧化酶（DAO）和D-乳酸的含量并检测肠黏膜上皮细胞凋亡率，对其做病理切片，评估其屏障功能。结果与囊型包虫病模型组比较，囊型包虫病致敏组TNF-α、IL-6、p-NF-κBp65含量及血浆中DAO和D-乳酸含量增加（P ＜ 0.05）。与囊型包虫病模型组比较，囊型包虫病致敏组肠黏膜明显萎缩，部分肠绒毛变短或断裂、脱落，间质较多急慢性炎症细胞浸润，肠黏膜上皮细胞凋亡增多。结论囊型包虫病所致过敏反应可引起肠黏膜损伤，导致肠黏膜屏障功能障碍。

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	商海波李鑫刘可可王江▲

关键词 ：囊型包虫病, 过敏反应, 肠黏膜屏障, 炎症因子

Abstract：Objective To investigate the effect of allergic reaction induced by echinococcosis granulosa on intestinal mucosal barrier function. Methods Forty-five female BALB/c mice aged six to eight weeks with SPF grade and body weight of 18 to 22 g were divided into blank control group, echinococcosis granulosa model group and echinococcosis granulosa sensitization group by random number table method, with 15 mice in each group. Echinococcus granulosus microcapsules were intraperitoneally injected into mice of echinococcosis granulosa model group and echinococcosis granulosa sensitization group. Each mouse was inoculated with about 50 microcapsules to establish the hydatid disease mouse model. After feeding for three months, the echinococcosis granulosa sensitization group was injected with crude hydatid cyst fluid to induce allergic reaction, while the blank control group and the echinococcosis granulosa model group were injected with the same amount of normal saline. The contents of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and phosphorylated nuclear factor of kappa B-p65 (p-NF-κBp65), diamine oxidase (DAO) and D-lactic acid in plasma and the apoptosis rate of intestinal mucosal epithelial cells were detected in intestinal tissue of mice in each group. Pathological sections were performed to evaluate the barrier function. Results Compared with echinococcosis granulosa model group, the contents of TNF-α, IL-6, p-NF-κBp65, and the contents of DAO and D-lactic acid in plasma were increased in echinococcosis granulosa sensitization group (P ＜ 0.05). Compared with echinococcosis granulosa model group, intestinal mucosa of echinococcosis granulosa sensitization group was significantly atrophied, part of intestinal villi became shorter or broke off, more acute and chronic inflammatory cells were infiltrated in the interstitium, and intestinal mucosa epithelial cells apoptosis increased. Conclusion Allergic reaction induced by echinococcosis granulosa can cause intestinal mucosal injury and lead to intestinal mucosal barrier dysfunction.

Key words： Echinococcosis granulosa Allergic reaction Intestinal mucosal Inflammation factors

基金资助:国家自然科学基金资助项目（81760044）。

通讯作者: ▲通讯作者

引用本文:

商海波李鑫刘可可王江▲. 囊型包虫病所致过敏反应对肠黏膜屏障功能的影响[J]. 中国医药导报, 2021, 18(16): 9-13.
SHANG Haibo LI Xin LIU Keke WANG Jiang▲. Effect of allergic reaction induced by echinococcosis granulosa on intestinal mucosal barrier function. 中国医药导报, 2021, 18(16): 9-13.

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