Correlation between MTHFR gene C677T and A1298C polymorphisms and plasma homocysteine in recurrent abortion
ZHAO Ai1 ZHANG Li1 MENG Lin2
1.Department of Obstetrics, Huai’an First People’s Hospital, Jiangsu Province, Huai’an 223300, China;
2.Department of Obstetrics, Affiliated Hospital of Xuzhou Medical University, Jiangsu Province, Xuzhou 221000, China
Abstract：Objective To investigate the correlation between MTHFR gene C677T and A1298C polymorphisms and plasma homocysteine in recurrent abortion. Methods A total of 100 women with recurrent abortion admitted to Huai’an First People’s Hospital, Jiangsu Province were selected as the recurrent abortion group, and 100 women with a history of one childbirth without history of adverse pregnancy were selected as the control group from December 2016 to June 2019. Restriction fragment length polymorphisms were used to detect the MTHFR gene C677T and A1298C genotypes; the occurrence risk of recurrent abortion with different genotypes was analyzed; colorimetric method was used to detect homocysteine level; the differences in homocysteine level between different genotypes were compared. Results There were statistically significant differences in the genotypes of C677T and A1298C between the two groups (all P < 0.05). Compared with C677T CC type, C677T TT type had a 3.375-fold increased risk of recurrent abortion (P = 0.008); Compared with A1298C AA type, A1298C CC had a 3.721-fold increased risk of recurrent abortion (P = 0.012). The homocysteine level in the recurrent abortion group was higher than that in the control group, and the difference was statistically significant (P < 0.05). Homocysteine level of C677T TT type was higher than that C677T CC type and C677T CT type, and the difference was statistically significant (P < 0.05); homocysteine level of A1298C CC type was higher than that of A1298C AA type and A1298C AC type, and the difference was statistically significant (P < 0.05). Conclusion The MTHFR gene C677T TT type and A1298C CC type have a higher risk of recurrent abortion, and C677T and A1298C polymorphisms can lead to elevated homocysteine level. The MTHFR gene C677T and A1298C mutations may be involved in the pathogenesis of recurrent abortion by changing homocysteine level.
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