Study of the effect of microcapsule fluidized culture on C3A cell function and its molecular mechanism
LI Xiaoqing1 LI Jianzhou2,3 YANG Ying3 LI Lanjuan3
1.Department of Emergency, First Affiliated Hospital of Xi’an JiaoTong University, Shaanxi Province, Xi’an 710061, China;
2.Department of Infectious Diseases, First Affiliated Hospital of Xi’an JiaoTong University, Shaanxi Province, Xi’an 710061, China;
3.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Zhejiang Province, Hangzhou 310003, China
Abstract：Objective To investigate the effect of microcapsule fluidized culture on C3A cell function and its molecular mechanism. Methods Base on the different culture modes of C3A cells, they were divided into three groups: two-dimensional culture group, microcapsule static culture group and microcapsule fluidized culture group. The effect of microencapsulation fluidized culture on the activity of C3A cells, albumin, urea synthesis capacity and metabolism ability was evaluated. The expression level of genes related to hepatocyte function was detected. The expression levels of key molecules in metabolism-related signaling pathways of hepatocytes were detected. Results The albumin, urea synthesis capacity and CYP1A2, CYP3A4 metabolic activity of C3A cells in the microcapsule static culture group and microencapsulation fluidized culture group were higher than those in the two-dimensional culture group, and the differences were statistically significant (P ＜ 0.05). Compared with the microcapsule static culture group, the albumin, urea synthesis capacity and CYP1A2 metabolic activity of C3A cells in the microcapsule fluidized culture group were increased, and the differences were statistically significant (P ＜ 0.05). The expression levels of CYP1A2, CYP2B62, CYP2E1, CYP3A4 and UGT2B4 genes of C3A cells in the microcapsule static culture group and microencapsulation fluidized culture group were higher than those in the two-dimensional culture group, and the differences were statistically significant (P ＜ 0.05). Compared with the microcapsule static culture group, the expression levels of p-PKC, p-PKA and calmodulin (CaM) proteins related to metabolism of C3A cells in the microcapsule fluidized culture group were decreased, and the differences were statistically significant (P ＜ 0.05). Conclusion Microcapsule fluidized culture can significantly improve the synthesis and metabolism fuction of C3A cells, and its mechanism of action may be related to the down-regulation of the phosphorylation level of related signaling pathway proteins and level of CaM.
李晓青1 李建州2,3 杨英3 李兰娟3. 微囊流化培养对C3A细胞功能的影响及其机制研究[J]. 中国医药导报, 2021, 18(15): 4-8,26.
LI Xiaoqing1 LI Jianzhou2,3 YANG Ying3 LI Lanjuan3. Study of the effect of microcapsule fluidized culture on C3A cell function and its molecular mechanism. 中国医药导报, 2021, 18(15): 4-8,26.
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