Abstract：Objective To prepare the Venenum Bufonis Chitosan Microspheres with deacetylated chitosan as a carrier and study the characteristics of Venenum Bufonis Chitosan Microspheres sustained release in vitro. Methods The Venenum Bufonis Chitosan Microspheres were prepared by emulsification-crosslinking method and their components were identified by thin layer chromatography. High performance liquid chromatography was used to detect the content of cinobufagin and resibufogenin and to detect the encapsulated efficiency of microspheres (the amount of drug encapsulated in the system divided by total dose of encapsulated and unencapsulated). The ultraviolet spectrophotometer was used to detect the indole alkaloids. Results The prepared microspheres had smooth surface and an average particle size of 9.20 μm. The three batches of microspheres showed fluorescence spots corresponding to the control substance and were negative without interference. The total drug loading of cinobufagin and resibufogenin, encapsulation rate and indole alkaloids content of microspheres were 6.06%, 73.30% and 6.59% respectively. Conclusion The prepared Venenum Bufonis Chitosan Microspheres have a good sustained release effect.
刘莉1 李宗云2 何世学2 赵越2 张春宝2. 蟾酥壳聚糖微球的制备及体外释药性研究[J]. 中国医药导报, 2021, 18(13): 31-34,46.
LIU Li1 LI Zongyun2 HE Shixue2 ZHAO Yue2 ZHANG Chunbao2. Study on preparation of Venenum Bufonis Chitosan Microspheres and sustained release characteristics in vitro. 中国医药导报, 2021, 18(13): 31-34,46.
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