Study on the effect and mechanism of Bixie Qufeng Decoction on gouty arthritis rats with dampness-heat amassment pattern
QIU Qingzhong1 QI Zirong2 XING Zhenlong1
1.Department of Joint and Trauma Orthopedics, Guangdong Integrated Traditional Chinese and Western Medicine Hospital, Guangdong Province, Foshan 528200, China;
2.Hand and Foot Surgery, Guangdong Integrated Traditional Chinese and Western Medicine Hospital, Guangdong Province, Foshan 528200, China
Abstract：Objective To explore the effect and mechanism of Bixie Qufeng Decoction on gouty arthritis rats with damp- ness-heat amassment pattern. Methods Fifty rats were divided into blank control group, model group, Colchicine group, high-dose Bixie Qufeng Decoction group and low-dose Bixie Qufeng Decoction group according to random number table method, with 10 rats in each group. The blank control group was fed normally, and the model group, Colchicine group, high-dose Bixie Qufeng Decoction group and low-dose Bixie Qufeng Decoction group were used to make the model of gouty arthritis with dampness-heat amassment pattern in rats by double modeling method. After the model was successfully established, the high-dose and low-dose Bixie Qufeng Decoction group were given high-dose and low-dose Bixie Qufeng Decoction extract by gavage (8.31, 4.52 g/kg) respectively, the Colchicine group was given Colchicine solution by gavage (at 9 mg/kg), and the model group and the blank control group were given equal volume of normal saline. The changes of joint swelling degree were observed before and after modeling, and the levels of uric acid (UA), interleukin (IL)-1β and cysteine aspartate (Caspase-1) were assessed by blood sampling. Results The joint swelling degree of the high-dose Bixie Qufeng Decoction group was lower than that of the model group, and the difference was statistically significant (P ＜ 0.05). Compared with the model group, the serum levels of UA, IL-1β and Caspase-1 in the high-dose Bixie Qufeng Decoction group were decreased, while the serum UA level in the low-dose Bixie Qufeng group were decreased, with statistical significance (all P ＜ 0.05). Conclusion In the treatment of gouty arthritis with dampness-heat amassment pattern, Bixie Qufeng Decoction may alleviate the inflammatory response of gouty arthritis by inhibiting the inflammatory mediators such as IL-1β and Caspase-1, and reduce the UA level of the body.
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