Roles of IL-17 signaling pathway in the development of relapsing fever neuroleptospirosis
CHENG Meihui LU Wei ZHANG Jing DING Kaiyun LIU Hongqi▲
Infection and Immunity Laboratory, Institute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Province, Kunming 650118, China
Abstract：Objective To investigate the roles of interleukin (IL)-17 signaling pathway in the development of relapsing fever neuroborreliosis. Methods Fourteen C57BL/6 wild-type mice were divided into two groups, six mice in group S, eight mice in group E, and eight mice with IL-17-/- signaling pathway defect (IL-17-/-) were IL-17-/- group. Three groups of mice were infected by intraperitoneal injection of DAH strain of Borrelia recurrentis (B.recurrentis), and the occurrence of bacteremia in vivo, specific antibodies and the incidence of nervous system were observed. The pathological change of spinal cord was compared between group E and IL-17-/- group. Results The mice in group S were effectively replicated and amplified and bacteremia was induced, and spirochete specific IgG and IgM were produced, and the incidence of symptoms of central nervous system diseases reached 100%. The level of bacteremia in mice of group E and IL-17-/- group were basically the same, and the production of spirochete specific IgG and IgM could be induced in mice of group E and IL-17-/- group. The central nervous system disease scores of IL-17-/- group infected with B.recurrentis were lower than those of group E after 9-14 d of infection, and the differences were statistically significant (P < 0.05 or P < 0.01). The number of microglial cells in spinal cord tissue of mice in IL-17-/- group was less than that in group E. Conclusion IL-17 signaling pathway plays an important role in the development of central nervous system diseases induced by B.recurrentis infection.
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