Effects of Selenium-enriched Danggui Yinyanghuo Recipe on SOD, MDA and serum sex hormone in rat testis
LI Jintao1 LI Shijun2 SONG Zhangxin2 XIANG Kui2 ZHAO Hua2 CUI Yingdong2 TIAN Zhaohui3
1.Department of Medicine, Hubei Minzu University, Hubei Province, Enshi 445000, China;
2.Department of Urology and Andrology, the National Hospital of Enshi Tujia and Miao Autonomous Prefecture, Hubei Province, Enshi 445000, China;
3.Department of Medical Administration, the National Hospital of Enshi Tujia and Miao Autonomous Prefecture, Hubei Province, Enshi 445000, China
Abstract：Objective To study the effects of different doses of Selenium-enriched Danggui Yinyanghuo Recipe on superoxide dismutase (SOD), malondialdehyde (MDA) and serum sex hormones (follicle stimulating hormone [FSH], luteinizing hormone [LH] and testosterone [T]) in rats testis. Methods Ten male SD rats were randomly selected as the normal group (gavage physiological saline), and the remaining rats were divided into four groups according to random number table method: model control group (gavage adenine), Selenium-enriched Danggui Yinyanghuo Recipe high dose group (gavage adenine + 15 days later, gavage 20.92 g/[kg·d] selenium-enriched traditional Chinese medicine), middle dose group (gavage adenine + 15 days later, gavage 10.46 g/[kg·d] selenium-enriched traditional Chinese medicine), low dose group (gavage adenine + 15 days later, gavage 5.23 g/[kg·d] selenium-enriched traditional Chinese medicine). The experiment lasted for 45 days with 12 rats in each group. The general state, SOD and MDA of testis, serum sex hormone and testicular morphology of rats were detected and compared in each group. Results Observation of general state: from the fourth day of modeling, the model control group and the Selenium-enriched Danggui Yinyanghuo Recipe groups showed clinical manifestations of reduced diet, decreased body weight and increased urine volume. After the implementation of drug intervention, the diet and body weight of the low, medium and high dose groups of Selenium-enriched Danggui Yinyanghuo Recipe gradually increased, and the urine volume and other clinical manifestations gradually tended to be normal. Compared with normal group, SOD decreased and MDA increased in testicular homogenate of model control group, with high statistical significance (all P < 0.01). Compared with the model control group, SOD in the Selenium-enriched Danggui Yinyanghuo Recipe high dose and medium dose groups increased to varying degrees, with high statistical significance (all P < 0.01). However, there was no significant difference between Selenium-enriched Danggui Yinyanghuo Recipe low dose group and model control group (P ＞ 0.05). Compared with the model control group, MDA in the high, medium and low dose groups of Selenium-enriched Danggui Yinyanghuo Recipe was decreased to different degrees, with high statistical significance (all P ＜ 0.01). Compared with normal group, serum FSH and LH were increased in model control group, while serum T was decreased, with high statistical significance (all P ＜ 0.01). Compared with model control group, serum FSH and LH were decreased and serum T was increased in the high and medium dose groups of Selenium-enriched Danggui Yinyanghuo Recipe, with high statistical significance (all P ＜ 0.01). Histopathology of testis showed obvious testicular injury in model control group, and improvement in low, medium and high dose groups of Selenium-enriched Danggui Yinyanghuo Recipe. Conclusion Selenium-enriched Danggui Yinyanghuo Recipe can improve the levels of SOD, MDA and serum sex hormones in the testis of rats after adenine modeling, and has a certain therapeutic effect on the testis injury caused by adenine.
李金涛1 李时军2 宋章兴2 向奎2 赵华2 崔应东2 田朝晖3. 富硒当归淫羊藿方对大鼠睾丸SOD、MDA及血清性激素的影响[J]. 中国医药导报, 2021, 18(4): 9-13,32.
LI Jintao1 LI Shijun2 SONG Zhangxin2 XIANG Kui2 ZHAO Hua2 CUI Yingdong2 TIAN Zhaohui3. Effects of Selenium-enriched Danggui Yinyanghuo Recipe on SOD, MDA and serum sex hormone in rat testis. 中国医药导报, 2021, 18(4): 9-13,32.
[1] Olayemi FO. A review on some causes of male infertility [J]. Afr J Biotechnol，2010，9（20）：2834-2842.
[2] Naz M，Kamal M. Classification causes diagnosis and treatment of male infertility：a review [J]. Orient Pharm Exp Med，2017，17（2）：89-109.
[3] 陈小均，王思师，贾玉森，等.男性不育症实验动物模型在中医药领域的应用概况[J].北京中医药，2018，37（3）：282-287.
[4] 秦川.实验动物学[M].北京：人民卫生出版社，2010：420.
[5] 宣玲，包玉婷，周小杰，等.肾阳虚大鼠肾组织中microRNA差异性表达与信号通路分析[J].中国实验方剂学杂志，2019，25（17）：57-63.
[6] Adachi Y，Sasagawa I，Tateno T，et al. Influence of adenine-induced chronic renal failure on testicular function in the rat [J]. Andrologia，1998，30（2）：115-118.
[7] Mazumder MK，Phukan BC，Bhattacharjee A，et al. Disturbed purine nucleotide metabolism in chronic kidney disease is a risk factor for cognitive impairment [J]. Med Hypotheses，2018，111：36-39.
[8] Yu ZZ, Chen J, Shou PQ，et al. Effects of micronutrients on the reproduction of infertility rat model induced by adenine [J]. Int J Clin Exp Med，2014，7（9）：2754-2762.
[9] 陈颖颖，罗静，徐愿，等.肾阳虚动物模型造模方法评价及进展[J].中华中医药学刊，2018，36（11）：2697-2700.
[10] 郑龙飞，韩玲，郝宇，等.金匮肾气丸加味膏、汤方干预肾阳虚模型大鼠的药效学对比研究[J].北京中医药大学报，2018，41（7）：567-571.
[11] Chen J，Yang Y，Zhu H，et al. Identification of differentially expressed SMADs in the testis of adenine-induced male infertile rats [J]. Caryologia，2014，67（3）：209-215.
[12] Agarwal A，Makker K，Sharma R，et al. REVIEW ARTICLE：Clinical Relevance of Oxidative Stress in Male Factor Infertility：An Update [J]. Am J Reprod Immunol，2008， 59（1）：2-11.
[13] Said TM，Agarwal A，Sharma R，et al. Human sperm superoxide anion generation and correlation with semen quality in patients with male infertility [J]. Fertil Steril，2004，82（4）：871-877.
[14] Aitken J，Krausz C，Buckingham DW，et al. Relationships between biochemical markers for residual sperm cytoplasm，reactive oxygen species generation，and the presence of leukocytes and precursor germ cells in human sperm suspensions [J]. Mol Reprod Dev，1994，39（3）：268-279.
[15] Kefer JC，Agarwal A，Sabanegh E，et al. Role of antioxidants in the treatment of male infertility [J]. Int J Urol，2009，16（5）：449-457.
[16] Walczak-Jedrzejowska R, Wolski JK, Slowikowska-Hilczer J. The role of oxidative stress and antioxidants in male fertility [J]. Cent European J Urol，2013，66（1）：60-67.
[17] Boitani C，Puglisi R. Selenium，a Key Element in Spermatogenesis and Male Fertility [J]. Adv Exp Med Biol，2008（636）：65-73.
[18] 田朝晖，崔应东，宋章兴，等.手术联合复方富硒当归淫羊藿合剂对精液质量的影响[J].临床与病理杂志，2018， 38（6）：1223-1227.
[19] 杨超，崔应东，廖兆琳，等.针挑联合复方富硒当归淫羊藿合剂对精液质量和精子顶体酶活性的影响[J].中国医药导报，2019，16（6）：54-57.
[20] 占心佾，李钰婷.香附、当归及白芍提取物方剂氧化损伤小鼠抗氧化功效研究[J].按摩与康复医学，2019，10（7）：47-49.
[21] 刘琛，黄淑娴，赖珊珊，等.加味当归芍药散对高催乳素血症大鼠环磷酸腺苷和蛋白激酶A及多巴胺受体水平的影响[J].中国医药，2019，14（2）：297-300.
[22] 赵文静，王历，王芝兰，等.淫羊藿的药理作用及临床应用研究进展[J].中医药信息，2016，33（2）：105-108.
[23] 徐薇，庄田畋.淫羊藿及相关配伍在良性前列腺增生症治疗中的应用现状[J].湖南中医杂志，2018，34（5）：209-211.
[24] 许国彬，徐仙，贾韶彤，等.菟丝子对弱精子症体外精子膜功能及顶体酶活性的影响[J].时珍国医国药，2011， 22（7）：1670-1672.
[25] 王惠洁.五子衍宗丸各药中治疗弱精症的化学成分及药理作用研究概况[J].山西中医学院学报，2017，18（4）：67-69.
[26] 刘文，章尹岗，别志霞，等.黄芪甲苷对肥胖糖尿病大鼠肾脏氧化应激、Nrf2蛋白表达和胰岛β细胞的影响[J].疑难病杂志，2019，18（1）：67-71，109.
[27] Wang Y，Lin C，Ren Q，et al. Astragaloside effect on TGF-β1，SMAD2/3，and α-SMA expression in the kidney tissues of diabetic KKAy mice [J]. Int J Clin Exp Pathol，2015，8（6）：6828-6834.
[28] 陈启云，蒋锦琴，褚建波，等.黄芪多糖对沙门菌诱导雄性大鼠睾丸损伤的保护作用[J].华西药学杂志，2019， 34（2）：161-164.
[29] 李强，曹陈军，陈奕，等.对丹参素药理作用的研究进展[J].当代医药论丛，2019，17（10）：16-18.
2021, Vol. 18 
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