Abstract：Objective To observe the effects of the optimized composition of Danggui Shaoyao San combined with the Mongolian medicine Chipaozi on serum inflammatory factors and histopathology in chronic pelvic inflammatory disease (CPID) model rats. Methods Seventy female SD rats were randomly divided into seven groups, including normal group, model group, high, medium, and low-dose Xinfang group, Jingangteng group, and Mongolian medicine Chipaozi group with 10 cases in each group. The CPID model was reproduced by the method of bacterial planting and mechanical damage, and medication was administered four weeks later. The high, medium, and low-dose groups of the new prescription were given by intragastric administration at 19.74, 9.87 g/kg, and 4.93 g/kg, respectively. The Jingangteng group and the Mongolian medicine Chipaozi group were given by oral administration at 0.63 g/kg and 1.04 g/kg. The material was taken two weeks after the administration. Enzyme-linked immunosorbent assay (ELISA) was used to determine serum tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-10 levels in rats in each group. The changes of rat uterine tissue under a microscope was observed and the pathology was scored. Results Compared with the normal group, the uterine pathology score of the model group was significantly increased, and the difference was highly statistically significant (P ＜ 0.01). The pathological score of Jingangteng group, high and medium Xinfang group were lower than that of model group, and the differences were statistically significant (P ＜ 0.05). Compared with the model group, the levels of IL-10 in the high and medium-dose Xinfang groups were increased, while the levels of IL-1β and TNF-α in the high, medium and low-dose Xingfang groups were decreased, and the differences were statistically significant (P ＜ 0.05). Compared with Jingangteng group, the content of TNF-α in the high and medium-dose Xinfang groups were decreased, and the differences were statistically significant (P ＜ 0.05). Compared with the Mongolian medicine Chipaonzi group, IL-10 in the high and medium-dose Xinfang groups were increased and IL-1β were decreased, and the differences were statistically significant (P ＜ 0.05). Conclusion The optimized prescription of Danggui Shaoyao San can counteract the abnormal expression of inflammatory cytokines in CPID model rats and improve the local pathological environment of model rats. The new prescription can promote the recovery of immune function by regulating the levels of TNF-α, IL-1β and IL-10 inflammatory factors, thereby exerting an active therapeutic effect on CPID model rats.
刘敏 王小燕 贾瑞琳 任存霞. 当归芍药散优化方抗慢性盆腔炎的实验研究[J]. 中国医药导报, 2021, 18(3): 17-20.
LIU Min WANG Xiaoyan JIA Ruilin REN Cunxia. Experimental study on the optimized prescription of Danggui Shaoyao San against chronic pelvic inflammation. 中国医药导报, 2021, 18(3): 17-20.
2021, Vol. 18 
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