miR-221/222靶向抑制对三阴性乳腺癌MDA-MB-231细胞顺铂敏感性的影响

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摘要目的探讨微RNA（miR）-221/222靶向抑制对三阴性乳腺癌（TNBC）MDA-MB-231细胞顺铂（DDP）敏感性的影响。方法随机将MDA-MB-231细胞分为对照组、miR-221抑制组、miR-222抑制组和miR-221/222抑制组，分别转染空白试剂、miR-221抑制剂、miR-222抑制剂和miR-221/222抑制剂，转染后均给予DDP 5 μg/mL进行培养。以荧光定量聚合酶链式反应检测miR-221/222 mRNA的相对表达量，以MTT法检测细胞增殖抑制率，以Annexin V-FTTC/PI双染法检测细胞凋亡率，以蛋白质印迹法分析凋亡相关蛋白的水平。结果 miR-221 mRNA的表达水平比较，miR-221抑制组低于对照组，miR-222抑制组高于miR-221抑制组，miR-221/222抑制组低于miR-222抑制组，差异均有统计学意义（均P ＜ 0.05）；miR-222抑制组和miR-221/222抑制组miR-222 mRNA的表达水平低于miR-221抑制组和对照组，差异均有统计学意义（均P ＜ 0.05）；miR-221/222抑制组转染后24、48 h的细胞增殖抑制率及细胞凋亡率均高于其余三组，差异均有统计学意义（均P ＜ 0.05）；miR-221/222抑制组转染后48 h细胞Caspase-9和Bax水平高于其余三组，Bcl-2水平低于其余三组，差异均有统计学意义（均P ＜ 0.05）。结论共抑制miR-221/222可增加MDA-MB-231细胞的DDP敏感性。

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	叶惠荣袁惠玲曹茵王西跃吴丽华陈桂林陈丽娟张玉娟▲

关键词 ：三阴性乳腺癌, 顺铂, miR-221, miR-222, 敏感性

Abstract：Objective To investigate the Cisplatin (DDP) sensitivity of microRNA (miR)-221/222 targeted inhibition on triple-negative breast cancer (TNBC) MDA-MB-231 cells. Methods MDA-MB-231 cells were randomly divided into control group, miR-221 inhibition group, miR-222 inhibition group and miR-221/222 inhibition group, transfected with blank reagent, miR-221 inhibitor, miR-222 inhibitor and miR-221/222 inhibitor respectively, and after transfection, DDP 5 μg/mL was given for culture. Relative expression of miR-221/222 mRNA was detected by fluorescence quantitative polymerase chain reaction, inhibition rate of cell proliferation was detected by MTT method, apoptosis rate was detected by Annexin V-FTTC/PI double staining method, and the expression levels of apoptosis related proteins were detected by Western blot. Results Comparing the expression level of miR-221 mRNA, the miR-221 inhibition group was lower than the control group, the miR-222 inhibition group was higher than the miR-221 inhibition group, and the miR-221/222 inhibition group was lower than the miR-222 inhibition group, and the differences were statistical significance (all P ＜ 0.05). The expression level of miR-222 mRNA in the miR-222 inhibition group and miR-221/222 inhibition group were lower than that of the miR-221 inhibition group and the control group, and the differences were statistically significant (all P ＜ 0.05). The inhibition rate of cell proliferation and apoptosis of the miR-221/222 inhibition group was higher than that of the other three groups at 24 and 48 h after transfection, and the differences were statistically significant (all P ＜ 0.05). The levels of Caspase-9 and Bax in the miR-221/222 inhibition group were higher than those of the other three groups at 48 h after transfection, and the Bcl-2 level was lower than that of the other three groups, and the differences were statistically significant (all P ＜ 0.05). Conclusion Co-inhibition of miR-221/222 can increase DDP sensitivity of MDA-MB-231 cells.

Key words： Triple-negative breast cancer Cisplatin miR-221 miR-222 Sensitivity

基金资助:国家自然科学基金资助项目（81802625）；
广东省东莞市市社会科技发展（重点）项目（2018507150011656）。

通讯作者: ▲通讯作者

引用本文:

叶惠荣袁惠玲曹茵王西跃吴丽华陈桂林陈丽娟张玉娟▲. miR-221/222靶向抑制对三阴性乳腺癌MDA-MB-231细胞顺铂敏感性的影响[J]. 中国医药导报, 2020, 17(35): 23-26,41.
YE Huirong YUAN Huiling CAO Yin WANG Xiyue WU Lihua CHEN Guilin CHEN Lijuan ZHANG Yujuan▲. Effect of miR-221/222 targeted inhibition on Cisplatin sensitivity of triple-negative breast cancer MDA-MB-231 cells. 中国医药导报, 2020, 17(35): 23-26,41.

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