潜阳合剂抑制心肌成纤维细胞增殖的机制研究

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摘要目的探讨潜阳合剂抑制血管紧张素Ⅱ（Ang Ⅱ）诱导的心肌成纤维（RCF）细胞异常增殖的机制。方法将RCF细胞分成空白细胞组、Ang Ⅱ组、Ang Ⅱ+潜阳合剂组。Ang Ⅱ组选用经过Ang Ⅱ诱导24 h的RCF细胞异常增殖的细胞模型，Ang Ⅱ+潜阳合剂组选用经过Ang Ⅱ诱导后加入潜阳合剂干预的细胞模型。蛋白质印迹法分析并比较三组的转化生长因子-β1（TGF-β1）、胶原蛋白Ⅰ型（Collagen Ⅰ）、Collagen Ⅲ蛋白表达，定量聚合酶链反应检测并比较三组的TGF-β1、Collagen Ⅰ、Collagen Ⅲ基因表达。结果 Ang Ⅱ组TGF-β1、Collagen Ⅰ、Collagen Ⅲ蛋白表达水平高于空白细胞组（均P ＜ 0.01）。Ang Ⅱ+潜阳合剂组的TGF-β1、Collagen Ⅰ、Collagen Ⅲ蛋白表达水平低于Ang Ⅱ组（P ＜ 0.05或P ＜ 0.01）。Ang Ⅱ组的TGF-β1、Collagen Ⅰ、Collagen Ⅲ基因表达水平高于空白细胞组（均P ＜ 0.01）。Ang Ⅱ+潜阳合剂组的TGF-β1、Collagen Ⅰ、Collagen Ⅲ基因表达水平低于Ang Ⅱ组（P ＜ 0.05或P ＜ 0.01）。结论潜阳合剂可以通过降低TGF-β1、Collagen Ⅰ、Collagen Ⅲ基因和蛋白的表达来抑制RCF细胞增殖，改善心肌纤维化。

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	陈婕阮小芬王肖龙

关键词 ：潜阳合剂, 血管紧张素Ⅱ, 心肌成纤维细胞, 心肌纤维化

Abstract：Objective To discussed the mechanism of Qianyang Decoction inhibits the abnormal proliferation of cardiac fibroblasts (RCF) cells induced by angiotensin Ⅱ (Ang Ⅱ). Methods The RCF cells were divided into blank cell group, Ang Ⅱ group and Ang Ⅱ+Qianyang Decoction group. In the Ang Ⅱ group, a cell model with abnormal proliferation of cardiac fibroblasts induced by Ang Ⅱ for 24 h was selected, and the Ang Ⅱ+Qianyang Decoction group was a cell model that was induced by Ang Ⅱ and intervened with Qianyang Decoction. Western blot was used to analyze and compare the transforming growth factor-β1 (TGF-β1), Collagen Ⅰ type (Collagen Ⅰ), Collagen Ⅲ protein expression among three groups, and quantitative polymerase chain reaction was used to detect and compare the TGF-β1 and Collagen Ⅰ, Collagen Ⅲ genetic expression among three groups. Results The protein expression levels of TGF-β1, Collagen Ⅰ and Collagen Ⅲ in Ang Ⅱ group were higher than those in the blank cell group (all P ＜ 0.01). The protein expression levels of TGF-β1 and Collagen Ⅰ, Collagen Ⅲ in Ang Ⅱ+Qianyang Decoction group were lower than those in the Ang Ⅱ group (P ＜ 0.05 or P ＜ 0.01). The gene expressions levels of TGF-β1, Collagen Ⅰ and Collagen Ⅲ in Ang Ⅱ group were higher than those in the blank cell group (all P ＜ 0.01). The gene expression levels of TGF-β1, Collagen Ⅰ and Collagen Ⅲ in Ang Ⅱ+Qianyang Decoction group were lower than those in Ang Ⅱ group (P ＜ 0.05 or P ＜ 0.01). Conclusion Qianyang Decoction can inhibits the abnormal proliferation of RCF cells by reducing the gene and protein expression level of TGF-β1, Collagen Ⅰ and Collagen Ⅲ, hence alleviate heart muscle fibrosis.

Key words： Qianyang Decoction Angiotensin Ⅱ Cardiac fibroblasts Heart muscle fibrosis

基金资助:国家自然科学基金青年科学基金项目（81803885）；
上海市高级中西医结合人才计划项目[ZY（2018-2020）-RCPY-2004]；
上海市中医临床重点实验室（14DZ2273200）。

通讯作者: 王肖龙（1965.1-），男，硕士，主任医师，博士生导师；研究方向：中西医结合心血管内科。

作者简介: 陈婕（1990.12-），女，硕士；研究方向：中西医结合心血管内科。

引用本文:

陈婕阮小芬王肖龙. 潜阳合剂抑制心肌成纤维细胞增殖的机制研究[J]. 中国医药导报, 2020, 17(30): 4-7,11.
CHEN Jie RUAN Xiaofen WANG Xiaolong. Mechanism research of Qianyang Decoction inhibits the proliferation of cardiac fibroblasts. 中国医药导报, 2020, 17(30): 4-7,11.

链接本文:

http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2020/V17/I30/4

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