CXCL-13通过激活PI3K-Akt信号通路对人骨髓间充质干细胞迁移的影响

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摘要目的验证趋化因子-13（CXCL-13）通过激活PI3K-Akt信号通路对人骨髓间充质干细胞（hBMSC）迁移的影响。方法无任何刺激的hBMSCs为对照组；80 μmol/L CXCL-13刺激hBMSCs为CXCL-13组；hBMSCs先用25 nmol/L LY294002培养40 min，再添加80 μmol/L CXCL-13为PI3K抑制剂组；hBMSCs先用50 nmol/L Triciribine培养30 min，再添加80 μmol/L CXCL-13为Akt抑制剂组。细胞划痕实验和Transwell细胞迁移实验检测四组hBMSCs划痕面积闭合率和细胞迁移率；酶联免疫吸附试验检测四组人PI3K、Akt、P-Akt蛋白的表达。结果 CXCL-13组hBMSCs划痕面积闭合率和迁移率均高于对照组，PI3K抑制剂组和Akt抑制剂组hBMSCs划痕面积闭合率和迁移率均低于CXCL-13组（P ＜ 0.05或P ＜ 0.01）；CXCL-13组人PI3K、Akt、P-Akt蛋白含量均高于对照组，PI3K抑制剂组和Akt抑制剂组人PI3K、Akt、P-Akt蛋白含量低于CXCL-13组（均P ＜ 0.01）。结论 CXCL-13激活PI3K-Akt信号通路促进hBMSCs迁移。

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	沈雷李永涛孙石柱姚立杰王璐璐刘丹阳金海峰张善强

关键词 ：趋化因子-13, 人骨髓间充质干细胞, Akt信号通路, 细胞迁移, 归巢

Abstract：Objective To verify C-X-C motif chemokine ligand-13 (CXCL-13) effect on human bone marrow mesenchymal stem cells (hBMSC) migration by activating PI3K-Akt signaling pathway. Methods hBMSC without stimulation was control group. hBMSCs were stimulated with 80 μmol/L CXCL-13 as CXCL-13 group. hBMSCs were first cultured with 25 nmol/L LY294002 for 40 min, and then 80 μmol/L CXCL-13 were added as PI3K inhibitor group. hBMSCs were cultured with 50 μmol/L Triciribine for 30 min in advance and then 80 μmol/L CXCL-13 were added as Akt inhibitor group. Cell wound scratch assay and Transwell cell migration assay were used to detect the scratch area closure rate and cell migration rate of hBMSCs in four groups. The expression of human PI3K, Akt and P-Akt proteins in hBMSCs of four groups were detected by enzyme linked immunosorbent assay. Results The hBMSCs scratch area closure rate and cell migration rate in CXCL-13 group were higher than those in control group, the hBMSCs scratch area closure rate and cell migration rate in PI3K inhibitor group and Akt inhibitor group were lower than those in CXCL-13 group (P ＜ 0.05 or P ＜ 0.01). The protein contents of human PI3K, Akt and P-Akt in CXCL-13 group were higher than those in control group, the protein contents of human PI3K, Akt and P-Akt in PI3K inhibitor group and Akt inhibitor group were lower than those in CXCL-13 group(all P ＜ 0.01). Conclusion CXCL-13 activates the PI3K-Akt signaling pathway to promote the migration of hBMSCs.

Key words： C-X-C motif chemokine ligand-13 Human bone marrow mesenchymal stem cell Akt signaling pathway Cell migration Homing

基金资助:国家自然科学基金资助项目（81541137）；
黑龙江省齐齐哈尔市科学技术计划项目（SFGG-201765）；
齐齐哈尔医学科学院项目（QMSI2019Z-18）；
齐齐哈尔医学院院内科研基金项目（QY2017Z-01）。

作者简介: 沈雷（1975-），男，博士，教授，硕士生导师，主要从事干细胞组织工程与再生医学研究。

引用本文:

沈雷李永涛孙石柱姚立杰王璐璐刘丹阳金海峰张善强. CXCL-13通过激活PI3K-Akt信号通路对人骨髓间充质干细胞迁移的影响[J]. 中国医药导报, 2020, 17(27): 4-7.
SHEN Lei LI Yongtao SUN Shizhu YAO Lijie WANG Lulu LIU Danyang JIN Haifeng ZHANG Shanqiang. Effect of CXCL-13 on human bone marrow mesenchymal mtem cell migration by activating PI3K-Akt signaling pathway. 中国医药导报, 2020, 17(27): 4-7.

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