Abstract：Objective To establish an ultra high performance liquid chromatography-tandem mass spectrometry method for the determination of Bortezomib in rat plasma. Methods Plasma sample preparation was treated by protein precipitation. ColumWaters XBridge?誖 BEH C18 (2.5 μm, 50 mm × 2.1 mm) was used as the column. Acetonitrile and 10 mmol/L ammonium formate solution was used as the mobile phase, with gradient elute, the volume flow rate was 0.3 mL/min, the column temperature was maintained at at 35℃. Multi-reaction detection positive ion mode was used to analysis. Results The Bortezomib in the range of 0.5-100 ng/mL showed a good liner, the standard linear regression equation was Y = 14.41X-0.4490 (r = 0.9974). The lower limit of quantification was 0.5 ng/mL, and the results of in-day, day-time precision and accuracy, extraction recovery, matrix effect and stability of quality control samples with low, medium and high 3 mass concentrations were all good. Conclusion The method is accurate, sensitive and reproducible, and can be used in the pharmacokinetic study of Bortezomib in rats.
刘进1 柳珂2 蒋巍3 黄翠云3 邓易3 束畅3 张凤3. UHPLC-MS/MS法测定大鼠血浆中硼替佐米的含量[J]. 中国医药导报, 2020, 17(10): 22-25.
LIU Jin1 LIU Ke2 JIANG Wei3 HUANG Cuiyun3 DENG Yi3 SHU Chang3 ZHANG Feng3. Determination of Bortezomib in rat plasma by UHPLC-MS/MS method. 中国医药导报, 2020, 17(10): 22-25.
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